Typing_Method |
Tm-Shift
|
Marker_Class |
SOL
|
STS_primer_fwd |
ACGTGCAAGAAGCCAGAGAAAATC
|
STS_primer_rev |
CTTAGCTTGTCTGCGACACTGCC
|
amplicon_seq |
NATATTCTCAAAACCCTAGATACCAATTTTTCATTTTCACCATTAAAATTAGGAAAATTTTCATACTCATCTTCATCCTCTGATTGCTTTGAAACTTGACAACAAGGGCCATTTACTTCTCATAGAACTAATTAAGATCCTCTTTTGAGTTGGGGTTTTTAAAGCCAAAATACTCCTTCCGTTCCTTTTTTAGCTGCATTTTTCGTGTCAATTTGAGCAACTAGATTACATTAATNNAATATTTTAATATGAAAATCTTGATATTCAAAAACTATATAAAAAGTACTATAAAGTGCAAGTTTTTTCATATTAGTATGTTAAAAAATATATCTGTTAGTCAAAATTCATATTGTTTATAGGAANCTGTGACTACTAAAAAGGAATGGAGGGAGTAGCTGTTAAGCACTTTTTATAGTATTTGGGTAGAACTGNCTGAACTTTGACCTACATTTTAAGAATTTATTTAGTTCATATGAGTCCCCGGACCCTACTTGTGGGATTCCACCGGATATGTTGTTTATTTATTTCGTAGGAGTATGAGAAGTATTGCAACTTATATTATTTCTCATATAGTTTTCAAACATCAATATATAATTAGTCAAATTGACTCTCACGAAACAAAATGGTGACAAGTAAAAGTGAAGGGAGTGAGCATATTTTATTTCTTTATCCAAGAGAAATGAAAAGTTTTTACAACTAACCATTCATTTGTGTTCCCATCCAGGTATTTATCCTCTGTTTTAGTATTAATTGAATGTGGATTTTGGGAAAATCTAGTACTATTTGAATTNNCCCGACTCGAAGAGTATGTTTATCAATAATTGTTTCCTCTAAACTTCTAGGANGTTTCTGCATTNAGGTCATCATCCATTTTGCTTTAGNATTCTTCTTCTCTCATCTGGNTTTCCAGAATGGAGATAGACCAAGGGCTTAGTTTAGATGACTGGAANTTATGTAGTATATTTGGNTTGTCATTTTTGTTTNGGCGGTCAAANTGTAACCAAGGNCCAAATTTATTNNNNNNNNNNNNNNNNNNNNCATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCGGGAGAAGCTAGAGGTGGCTCGGTGGCCNGATAATATGAGGGAAGCGAGACTGCGATGGTTTGGACATGTGCAGAGAAGGAGTACTGATGGTCCTGTTAGGAGGCGCGAGAGGTTGGTTTTAGGAGCTACGCAGAGGGGTAGAGGTAGACCGAAGAAGTACTGGGGGGAGGTGATTAGACATGATATGGCGGAGCTTGATCTCACAGAGGACATGACCCTAGATAGGAAGNAGTGGAGGTTGCGAATTAGAGTAGTAGGCTAATTGGCAGGGTTAGGTGGATGCATGGGTTAGGTGGAGGACGTGTTTTCTCTTTTTGTGTTCTCATTATCTGTTTTATATGTGTTAGTTGTATCATATTTACATATTGCCATGTGCTATTCATTACTAGTCACTCTTTGTGTTGCCTTCCCTTTCCTTTTTGTCATATCTAGTCCTTTCTGCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACTTCTATGAGGTAGTGGTAAGGTTTGCGTACAGACCCCACTTGTGGGATTTTACTGGGTATGTTGTTGTTNTTGTAAAAGCTTTTTGATTAATTTTGCATTTCAACGTATATATAGCATGGTGTCACTTTAGGCGGGACGTGTCAATGGGCTCTTCACGGTGCCTTTTCCTGTCTCATAATCTCTTGTAAGAGTATTTGCTCAGAATTGAGAAAAAGTTCATTTCATGCTTTTGTGGAACAGGCCATGTGCATTGTGCATTGGCTTTGTTATGTCTAACTTTCTGTTCTCTTCCTTAAAGGTCACTTATGTTACATTGTGCGACTCTTTGTTATTACATATGCTATCTGACATCCGTGAATAACTGTCATGAAATTTTGACAAATATCTGTTATAGTTATTTGTGTCATCAGTTTTCCTTTCTACTTGAGCTTTTTNATGTATCAAATATTTCAGCTTTGCGCAGTAAAATGAAGCAGAAAGCACTCAAAGAATGTGATCGATATACTGCAAAATATGCTGAGTGTGCCTCNNNNNNNNNNNNNNNNNNN
|
SNP_position |
138
|
allele_1 |
T
|
allele_2 |
G
|
ASP_primer_S |
GCGGGCCTTTAAAAACCCCAACTCAAAAGAGGA
|
ASP_primer_L |
GCGGGCAGGGCGGCCTTTAAAAACCCCAACTCAAAAGATGC
|
Common_primer |
TTGAAACTTGACAACAAGGGCCATTTA
|
enzyme |
Native Taq
|
dye |
SYBR Green I
|