+Marker Data: emh01E24

Crop eggplant
Population AL2010
Linkage Group E_06
Marker Type SSR
Map Position 60.5
Annotation
Source Name emh01E24
Source Type genomic
Source Sequence ACTCTCGTCGCATTTCCTATCATGTTTCAACAAAGCAACA GTCAAGTTGAGAGAGAGAGAGAGAGAGAGAGAGAGAGTTG AAGCTTCCACTAGTTTGTCATATGGCCTAAGACTCTTGCT GGAAAAAGAGAACATGCATGATGAGCTAAATGAACATTTG AGCAGTGGCTCTCTCTCAGAAGAAGAGAGAATTTCTCAAA AAATTAAACTCAATTCTCAAGCGCTCGGTCTTGTTATGCA TAGAAGGGGTTATTTGGCCAAAACATACCCAAAAAAAAAT CAATTTTATAAACATAACGATCAGAAATGGTGTTATTGAC GTATGAACCAGCCAACAGACTGGAGCACAGGTGAAGTGTT TGGCTGCTGTTATGTAAATTTATAATCATTTCAGTGTTAC TGGAATAGAGACCAACAAAAGTAAATGTAAAATGTCAAAA GATTTCTTACAGAGGGGTAACTAGGCATATAGACGACTTG CCGAGGATGACCAATCATCATCTGCTGTCCATACTGCATT GAAAAGATAAGAATTTTGAACATTCTAAAACCCATTTCAA ACATAATGACAGAAGAATCAGT
Developer NIVTS
Document Nunome et al. (2009) Development of SSR markers derived from SSR-enriched genomic library of eggplant (Solanum melongena L.). Theor. Appl.Genet. 119: 1143-1153

motif (ag)14
PCR product (bp) 244
fwd primer emh01E24/U
fwd primer seq. cgtcgcatttcctatcatgtttca
rev primer emh01E24/L
rev primer seq. ccccttctatgcataacaagaccg
fluor. primer emh01E24/U_NED
dye NED
fluor. Primer seq. NED-agtcgcatttcctatcatgtttca
PCR condition 94C_3min->(94C_30s->65C_60s,-1C/cyc->72C_60s)x10->(94C_30s->55C_60s->72C_60s)x30->75C_5min->10C
polymerase Roche Taq

Line allele-1 allele-2
AE-P03 246 246
EPL-1 246 246
LS1151 (S.linneanum) 233 233
LS1934 264 264
LS4021 (S. incanum) 285 285
Nakate Shinkuro 246 246
Senryo 2 246 246
Shironasu 246 246
WCGR112-8 246 246
White Egg 260 260
PI 286106 262 262

Population Linkage Group Position
AL2010 E_06 60.5
LW2010 E_06 67.7
LWA2010 LWA_06 64.866
LWAE2012 E_06 70.455
LWF2_2022 E_06 82.0
N28F2 E_06 97.6

DB Program Definition E-Value

EST Name Species Strain