+Marker Data: emg21I17

Crop eggplant
Population AL2010
Linkage Group E_06
Marker Type SSR
Map Position 38.6
Annotation
Source Name emg21I17
Source Type genomic
Source Sequence GATAAATAATTCACTACAAATATTATTTTGATAAATTATT TATTTAGAAAGATCATGTATATCATGGTAAACTAGAATTT TTCTTAGTGACCGTAGAGTATTTTCTTGATTCAAATAAGG AAGAAGCAAAAGTCTATCAAATAAAGAGGATTTAGGAGTC TTTTAACAGCTACCTTATTTTATCATTTATTTTTATATAT TTTAAAAGTCAATATTATAAATATAAATAAATAAGAATTT AAACAAAAATGGTGGAAAGACAATTTTGTCTAAAGAGGAG TATTTTAATGAAGGCAAAAAAGTTCAAACAACATTTCTAA GGGCCTTCACGTTTTTAATATAATATAGATAATAATATAT TAAAATGTATTGTTGTTGTTGTTGTTGTTGTTGTTGTTGT TGTGTTGTTGTTGTTGTTGTTTAGTTATACATTCAACAAG TGCCAAATATGCCCAATTTGTAATATATTGAAATTAAAAT GTATTACAGTTATCACCCTTATTTTTTATTAACTATGTCA AAAAAATATTATATGTGAATTTCAAATGTTTAAGCAAAAT TTAAATTAATTTATATGTTATATATAAAGAGAGAGACAGA GAGAGAGAGAGAGAAATTTAATGTCTTTTATGTTGTTTAT ATGCTTCCGCGACCAATGATGTGTAATGATAAGTTAAGAG TCTTGTTTGGAGTTTCTTCGGGTTCTCCATTCGTCGTATT ACGTCTAGGATTTCTTTTTGATTATTCGATATTCAAAATT TATCGATCAACTAATTTGAATTACGTGATAAAAATCCACT ATAATTTTTTTTTTAATTCTTTAAACTAAAAAGGAATCTT TTTGAATCTTGATAATTGGATATTATTGAAGTAAAATTAA TAGGGCAGG
Developer NIVTS
Document Nunome et al. (2009) Development of SSR markers derived from SSR-enriched genomic library of eggplant (Solanum melongena L.). Theor. Appl.Genet. 119: 1143-1153

motif (at)3taaaatgtat(tgt)11gt(tgt)5
PCR product (bp) 147
fwd primer emg21I17/U
fwd primer seq. acaacatttctaagggccttcacg
rev primer emg21I17/L
rev primer seq. gggcatatttggcacttgttgaat
fluor. primer emg21I17/U_PET
dye PET
fluor. Primer seq. PET-acaacatttctaagggccttcacg
PCR condition 94C_3min->(94C_30s->65C_60s,-1C/cyc->72C_60s)x10->(94C_30s->55C_60s->72C_60s)x30->75C_5min->10C
polymerase Roche Taq

Line allele-1 allele-2
AE-P03 161 161
EPL-1 161 161
LS1151 (S.linneanum) 223 223
LS1934 189 189
LS4021 (S. incanum) 195 195
Nakate Shinkuro 162 162
Senryo 2 161 161
Shironasu 161 161
WCGR112-8 158 158
White Egg 161 161
PI 286106 196 196

Population Linkage Group Position
AL2010 E_06 38.6
EW2009 LG04 71.2
LW2010 E_06 35.7
LWA2010 LWA_06 36.986
LWAE2012 E_06 43.283
LWF2_2022 E_06 41.9
N28F2 E_06 49.4

DB Program Definition E-Value

EST Name Species Strain