+Marker Data: emf21I08

Crop eggplant
Population AL2010
Linkage Group E_06
Marker Type SSR
Map Position 38.6
Annotation
Source Name emf21I08
Source Type genomic
Source Sequence CTTGGTTTTCTAGAGAAAAGGTTTTCCAAAATATTTTGAC CAACCATAGGAAACTTGAAAAACAGTTCCCTCGTACCAAA CACGTTCTCAGAGCATTTTTACTTTCACATCTGACTAGTG ATTTAGGAAAGTAAGTGTTCCCTTTTCCTTCTTTCTGTTA CTGTTGTTTCTGGCCATTAGATAATACTTTTTCCCATTAG TTTTTGAGGGATTTTTCTATCCAAGGACCAAAAGTGTCCA CTCTTAATAACTAAAAGGATGTTCTTTATTCATATATATA TTTAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA GAGAGAGAGAGAAAACCAAAATACTTCTACAGCAATACTT AAGGGACTATTTTGTTTTTTTCCCCTCTATAGTGATTGTG CAAGATCACCTTTCTGACTGCCAAGAGAAATTAGAAGTTT ATGGAATCAATTGTTCTGTAACTTACTACTCTTCTCTAAT GGAAACTTATACCTGTTTCCTGAAA
Developer NIVTS
Document Nunome et al. (2009) Development of SSR markers derived from SSR-enriched genomic library of eggplant (Solanum melongena L.). Theor. Appl.Genet. 119: 1143-1153

motif (ta)4ttt(ag)24
PCR product (bp) 209
fwd primer emf21I08/U
fwd primer seq. tctatccaaggaccaaaagtgtcca
rev primer emf21I08/L
rev primer seq. ttggcagtcagaaaggtgatcttg
fluor. primer emf21I08/U_FAM
dye FAM
fluor. Primer seq. FAM-actatccaaggaccaaaagtgtcca
PCR condition 94C_3min->(94C_30s->65C_60s,-1C/cyc->72C_60s)x10->(94C_30s->55C_60s->72C_60s)x30->75C_5min->10C
polymerase Roche Taq

Line allele-1 allele-2
AE-P03 212 212
EPL-1 210 210
LS1151 (S.linneanum) 189 189
LS1934 210 210
LS4021 (S. incanum) 202 202
Nakate Shinkuro 208 208
Senryo 2 210 210
Shironasu 208 208
WCGR112-8 208 208
White Egg 212 212

Population Linkage Group Position
AL2010 E_06 38.6
EW2009 LG04 71.2
LW2010 E_06 35.6
LWA2010 LWA_06 36.954
LWAE2012 E_06 43.55
LWF2_2022 E_06 41.9

DB Program Definition E-Value
TomatoBAC_end BLASTN LE_HBa0011L15_T7_5654 Chromat_file:LE_HBa_011_L15-T7 SGN_GSS_ID:5654 (vector and quality trimmed) 1e-06

EST Name Species Strain