+Marker Data: emf21C11

Crop eggplant
Population AL2010
Linkage Group E_06
Marker Type SSR
Map Position 38.6
Annotation
Source Name emf21C11
Source Type genomic
Source Sequence GGTCATCCTTCAACACTTTTCCACGGCATATGAGCCGTTG TTGTTCTGTCAAGACACCTGTAACTGTGGCAATTTGTTCC TTCAATGCAGGAACTGGTACCTGTATTGGAATTGACAACA GGTATTAGAAAGAATTTTAGAATATTATAAAAGTCAAAAC AGATGATACTACAGTCAACCTTTAAATTCTTTTGATACTA CAGTCAACCTTTAAATTGTCAGTTAAAGAGCATGATTGGT CTTTGTAATTGTGCTCCCATATCCAACAAGGATGCACATT TGCAACTTACAGATACAGAAAACTGGTTGGAGCCATGATT ACTTGAAATTCTAGTCTCTCTCTCTCTCTCTCTCTCTCTC TCTCTCTCTCTCTCTCTCTCTCCCTTTTTTTTGGGGAGAT TAGTACTTGAAATTCTACTTTTTGAAGAAGTATATAAACA AATTACAAATAGAACAATCGGCCTTGATGTTTTTATTGTT CCGCCTGTTTGATAGGTAGCATAGCTCAAGAGGGAAGAAC CGATTAAAGAAGGATGATTTGAATTCCTCCCAAAGCACCA CATGGCCATTACCCTTCTCATCCTTCCATCGATCAAACTA AATTTAAGCCACCCCTTTGAGTTAGTACGCCGCCAACTCC ACTTTTTCATTTGGTCCAACACCCATGATAGATAAGATTT TTAGATTTCATCAATAAATTCTTATGGATCTTCATCCACC TTTGACCCATGAAAAATCAA
Developer NIVTS
Document Nunome et al. (2009) Development of SSR markers derived from SSR-enriched genomic library of eggplant (Solanum melongena L.). Theor. Appl.Genet. 119: 1143-1153

motif (tc)24
PCR product (bp) 199
fwd primer emf21C11/U
fwd primer seq. tggttggagccatgattacttgaa
rev primer emf21C11/L
rev primer seq. atgctacctatcaaacaggcggaa
fluor. primer emf21C11/U_PET
dye PET
fluor. Primer seq. PET-aggttggagccatgattacttgaa
PCR condition 94C_3min->(94C_30s->65C_60s,-1C/cyc->72C_60s)x10->(94C_30s->55C_60s->72C_60s)x30->75C_5min->10C
polymerase Roche Taq

Line allele-1 allele-2
AE-P03 205 205
EPL-1 205 205
LS1151 (S.linneanum) 199 199
LS1934 203 203
LS4021 (S. incanum) 203 203
Nakate Shinkuro 213 213
Senryo 2 205 205
Shironasu 211 211
WCGR112-8 201 201
White Egg 199 199
PI 286106 206 206

Population Linkage Group Position
AL2010 E_06 38.6
EW2009 LG04 71.2
LW2010 E_06 35.7
LWA2010 LWA_06 36.999
LWAE2012 E_06 43.602
LWF2_2022 E_06 41.9
N28F2 E_06 49.4

DB Program Definition E-Value
TomatoBAC_end BLASTN LE_HBa0044N02_T7_143080 Chromat_file:LE_HBa_044_N02-T7 SGN_GSS_ID:159911 (vector and quality trimmed) 1e-56
TomatoBAC_end BLASTN SL_MboI0025A21_T7_216969 Chromat_file:SL_MboI_25_A21-T7 SGN_GSS_ID:233795 (vector and quality trimmed) 7e-06

EST Name Species Strain