+Marker Data: emf01J09

Crop eggplant
Population AL2010
Linkage Group E_06
Marker Type SSR
Map Position 38.6
Annotation
Source Name emf01J09
Source Type genomic
Source Sequence GTTTAAGCCTTGCTAGCAGAAGCCTCACTCGTTTTGAAAT TAAGGGATAAAAGGTTATTGCACACATAGACAACAAACCA GACTATACTAAAAGAGAGATTAGTCGGAAACATCATATTC TTTATCCAATCTCACAGAAGGCGAATATCCTCTAGCTTTT TTATGAAAAGTATAGGTGTCTCTGTACTATTCCGCAAGAC TCTATTAGATTTATTTTTATACAATGAGATCAATGAAACA AAGCTTTGATACCAATTTTGTCACAGTCCCAAATAGAATG TGAGTAGCACCCACACTAAACCTTGGGAGAACTAATACTT TAATCCAACCACCAGAATAATATAATTCACACAAATTTTA AATTACGGAAGCAAATTTAAATAAGTAAAACAAGTTGAGT TTCCATAAACTTAAATAGAAACTTAACAACATAAAATATT GTGTGTGTGTGTGTGAGAGAGAGAGAGAGAGAGAGAGAGA GAGAGAGAAAGAGAGAGAAAGGGATCCAACCCTGAGAATT CATAATAAGGTAAAGTCTAAGAGTGTCTATTTCTGAACAA TGGACCAAGAAGTGAATTTGAATCTAGGTAGCCTGAACAA GCTAG
Developer NIVTS
Document Nunome et al. (2009) Development of SSR markers derived from SSR-enriched genomic library of eggplant (Solanum melongena L.). Theor. Appl.Genet. 119: 1143-1153

motif (tg)8(ag)16aa(ag)4
PCR product (bp) 293
fwd primer emf01J09/U
fwd primer seq. gtagcacccacactaaaccttggg
rev primer emf01J09/L
rev primer seq. ttcacttcttggtccattgttcaga
fluor. primer emf01J09/U_NED
dye NED
fluor. Primer seq. NED-atagcacccacactaaaccttggg
PCR condition 94C_3min->(94C_30s->65C_60s,-1C/cyc->72C_60s)x10->(94C_30s->55C_60s->72C_60s)x30->75C_5min->10C
polymerase Roche Taq

Line allele-1 allele-2
AE-P03 302 302
EPL-1 293 293
LS1151 (S.linneanum) 280 280
LS1934 289 289
LS4021 (S. incanum) 286 286
Nakate Shinkuro 302 302
Senryo 2 302 304
Shironasu 304 304
WCGR112-8 303 303
White Egg 293 293
PI 286106 300 300

Population Linkage Group Position
AL2010 E_06 38.6
EW2009 LG04 66.4
LW2010 E_06 35.6
LWA2010 LWA_06 36.965
LWAE2012 E_06 43.561
LWF2_2022 E_06 41.9
N28F2 E_06 53.1

DB Program Definition E-Value
TomatoBAC_end BLASTN SL_EcoRI0026P03_T7_232331 Chromat_file:SL_EcoRI_026_P03-T7 SGN_GSS_ID:249157 (vector and quality trimmed) 9e-08

EST Name Species Strain