+Marker Data: AFS111

Crop bunching_onion
Population DJF2
Linkage Group Chr2b
Marker Type SSR
Map Position 32.5
Annotation
Source Name AB499330
Source Type genomic
Source Sequence
Developer NIVTS
Document Song et al. (2004) Breed Sci 54:361-365
motif (at)3gtgtctcta(tg)8
PCR product (bp) 223
fwd primer AFS111-F
fwd primer seq. tgtttaatggactttcaatgcctgt
rev primer AFS111-R
rev primer seq. gcattaaaatgaagaaatcccgaag
fluor. primer AFS111-F(FAM)
dye FAM
fluor. Primer seq. FAM-tgtttaatggactttcaatgcctgt
PCR condition 94C_2min->(94C_30s->60C_30s,-1C/cyc->72C_30s)x10->(94C_30s->50C_30s->72C_30s)x35->72C_4min->10C
polymerase Toyobo rTaq DNA polymerase
Genebank No. AB499330
annotation
similar marker
product size in J 230
product size in Kaizuka-Wase 230
product size in Iki-White 200
product size in Tender Pole
Line allele-1 allele-2
C1s-11s-25s-7 228 228
Cho1s-1s-2s-2s-8s 228 228
D1s-15s-10s-8 228 228
F1s-7s-17s-3s-1 230 230
J1s-14s-23s-9 230 230
K1s-5s-2s-2s-4s-5 230 230
Chiang_mai 210 230
Kaizuka_Wase
Population Linkage Group Position
DJF2 Chr2b 32.5
KiCF2 2b 89.8
P1 J03 43.6
P2 D03 83.6
DB Program Definition E-Value
EST Name Species Strain