+Marker Data: AFS101

Crop bunching_onion
Population DJF2
Linkage Group Chr8
Marker Type SSR
Map Position 52.4
Annotation
Source Name AB499326
Source Type genomic
Source Sequence
Developer NIVTS
Document Tsukazaki et al. (2008) Theor Appl Genet 117:1213-1223

motif (ac)5atat(ac)9atatacag(at)3
PCR product (bp) 155
fwd primer AFS101-F
fwd primer seq. atcacacacacacatatacacacac
rev primer AFS101-R
rev primer seq. agcgaccatatatagccttgcctta
fluor. primer
dye
fluor. Primer seq.
PCR condition 94C_2min->(94C_30s->60C_30s,-1C/cyc->72C_30s)x10->(94C_30s->50C_30s->72C_30s)x35->72C_4min->10C
polymerase Toyobo rTaq DNA polymerase
Genebank No. AB499326
annotation
similar marker
product size in J
product size in Kaizuka-Wase
product size in Iki-White
product size in Tender Pole

Line allele-1 allele-2
C1s-11s-25s-7
Cho1s-1s-2s-2s-8s
D1s-15s-10s-8 159 159
F1s-7s-17s-3s-1
J1s-14s-23s-9 155 155
K1s-5s-2s-2s-4s-5
Chiang_mai
Kaizuka_Wase

Population Linkage Group Position
DJF2 Chr8 52.4
SaT03 8a 26.7

DB Program Definition E-Value

EST Name Species Strain