+Marker Data: AFRT01F02

Crop bunching_onion
Population DJF2
Linkage Group Chr7a
Marker Type SSR
Map Position 73.0
Annotation
Source Name AB499523
Source Type genomic
Source Sequence
Developer NIVTS
Document Tsukazaki et al. (2007) Euphytica 157:83-94

motif (cg)3c(ac)6
PCR product (bp) 216
fwd primer AFRT01F02-U
fwd primer seq. acgaggcataggtggatgaggtta
rev primer AFRT01F02-L
rev primer seq. gtttcagccaactcgtcataggaacgata
fluor. primer AFRT01F02-F(NED)
dye NED
fluor. Primer seq. NED-acgaggcataggtggatgaggtta
PCR condition 94C_2min->(94C_30s->65C_30s,-1C/cyc->72C_30s)x10->(94C_30s->55C_30s->72C_30s)x35->72C_4min->10C
polymerase Toyobo rTaq DNA polymerase
Genebank No. AB499523
annotation
similar marker
product size in J 220
product size in Kaizuka-Wase
product size in Iki-White
product size in Tender Pole

Line allele-1 allele-2
C1s-11s-25s-7 222 222
Cho1s-1s-2s-2s-8s 222 222
D1s-15s-10s-8 222 222
F1s-7s-17s-3s-1 222 222
J1s-14s-23s-9 220 220
K1s-5s-2s-2s-4s-5 222 222
Chiang_mai
Kaizuka_Wase

Population Linkage Group Position
DJF2 Chr7a 73.0
FA 7a 32.1
SaT03 7a 33.1

DB Program Definition E-Value

EST Name Species Strain