| motif |
(tg)4atgcgtgcga(tg)3cga(tg)4a(tg)3
|
| PCR product (bp) |
300
|
| fwd primer |
AFRA04F12-U
|
| fwd primer seq. |
agaggctcaggtgtagactcggtt
|
| rev primer |
AFRA04F12-L
|
| rev primer seq. |
gtttgcctcacaaatcacactcaaca
|
| fluor. primer |
AFRA04F12-F(PET)
|
| dye |
PET
|
| fluor. Primer seq. |
PET-agaggctcaggtgtagactcggtt
|
| PCR condition |
94C_2min->(94C_30s->65C_30s,-1C/cyc->72C_30s)x10->(94C_30s->55C_30s->72C_30s)x35->72C_4min->10C
|
| polymerase |
Toyobo rTaq DNA polymerase
|
| Genebank No. |
AB499518
|
| annotation |
|
| similar marker |
|
| product size in J |
300
|
| product size in Kaizuka-Wase |
|
| product size in Iki-White |
|
| product size in Tender Pole |
|
