+Marker Data: AFC01E09

Crop bunching_onion
Population DJF2
Linkage Group Chr7a
Marker Type SSR
Map Position 110.6
Annotation
Source Name AB499499
Source Type genomic
Source Sequence
Developer NIVTS
Document Tsukazaki et al. (2007) Euphytica 157:83-94

motif (tct)7
PCR product (bp) 143
fwd primer AFC01E09-U
fwd primer seq. accttttgttcgacaagatttcca
rev primer AFC01E09-L
rev primer seq. gtttcaagacagcgatgacaaggaagaa
fluor. primer AFC01E09-F(FAM)
dye FAM
fluor. Primer seq. FAM-accttttgttcgacaagatttcca
PCR condition 94C_2min->(94C_30s->65C_30s,-1C/cyc->72C_30s)x10->(94C_30s->55C_30s->72C_30s)x35->72C_4min->10C
polymerase Toyobo rTaq DNA polymerase
Genebank No. AB499499
annotation
similar marker
product size in J 140
product size in Kaizuka-Wase
product size in Iki-White
product size in Tender Pole

Line allele-1 allele-2
C1s-11s-25s-7 140 140
Cho1s-1s-2s-2s-8s 146 146
D1s-15s-10s-8 140 140
F1s-7s-17s-3s-1 143 143
J1s-14s-23s-9 146 146
K1s-5s-2s-2s-4s-5 140 140
Chiang_mai
Kaizuka_Wase

Population Linkage Group Position
DJF2 Chr7a 110.6
FA 7a 33.4
KiCF2 7a 53.7

DB Program Definition E-Value

EST Name Species Strain