motif |
(tg)3ctatgtgcacgtg(ta)4attgc(gt)3a(ta)3ag(tg)4cgttc(gt)3atatca(gt)3(at)3gacagatt(tg)3c(gt)3agtatacctgtatgttag(tg)4cgtgagtgtaattt(tg)5ttgc(gt)3atagg(ta)4aa(tg)8
|
PCR product (bp) |
300
|
fwd primer |
AFAT05G01-U
|
fwd primer seq. |
agtgtgttgcagtgtttgtgtatg
|
rev primer |
AFAT05G01-L
|
rev primer seq. |
gtttccactacaccgcacatccctatatc
|
fluor. primer |
AFAT05G01-F(FAM)
|
dye |
FAM
|
fluor. Primer seq. |
FAM-agtgtgttgcagtgtttgtgtatg
|
PCR condition |
94C_2min->(94C_30s->65C_30s,-1C/cyc->72C_30s)x10->(94C_30s->55C_30s->72C_30s)x35->72C_4min->10C
|
polymerase |
Toyobo rTaq DNA polymerase
|
Genebank No. |
AB499457
|
annotation |
|
similar marker |
|
product size in J |
300
|
product size in Kaizuka-Wase |
|
product size in Iki-White |
310
|
product size in Tender Pole |
310
|