+Marker Data: AFA03D07

Crop bunching_onion
Population DJF2
Linkage Group Chr6a
Marker Type SSR
Map Position 99.1
Annotation
Source Name AB499363
Source Type genomic
Source Sequence
Developer NIVTS
Document Tsukazaki et al. (2008) Theor Appl Genet 117:1213-1223

motif (tg)3c(ac)18
PCR product (bp) 293
fwd primer AFA03D07-U
fwd primer seq. agtcctcgaaaatgagatgcttca
rev primer AFA03D07-L
rev primer seq. gtttgaaatgtaacccgactgtccaaa
fluor. primer AFA03D07-F(FAM)
dye FAM
fluor. Primer seq. FAM-agtcctcgaaaatgagatgcttca
PCR condition 94C_2min->(94C_30s->65C_30s,-1C/cyc->72C_30s)x10->(94C_30s->55C_30s->72C_30s)x35->72C_4min->10C
polymerase Toyobo rTaq DNA polymerase
Genebank No. AB499363
annotation
similar marker
product size in J
product size in Kaizuka-Wase
product size in Iki-White
product size in Tender Pole

Line allele-1 allele-2
C1s-11s-25s-7
Cho1s-1s-2s-2s-8s 282 282
D1s-15s-10s-8 290 290
F1s-7s-17s-3s-1
J1s-14s-23s-9 310 310
K1s-5s-2s-2s-4s-5 302 302
Chiang_mai 197 214
Kaizuka_Wase

Population Linkage Group Position
DJF2 Chr6a 99.1
KiCF2 6a 378.0

DB Program Definition E-Value

EST Name Species Strain