| motif |
(tg)4tatg(ta)6tgtgg(tg)3(ta)6
|
| PCR product (bp) |
245
|
| fwd primer |
ACE127-F
|
| fwd primer seq. |
tcgagacgagcacgcatgaaaa
|
| rev primer |
ACE127-R
|
| rev primer seq. |
agctccattgtccgttccatt
|
| fluor. primer |
|
| dye |
|
| fluor. Primer seq. |
|
| PCR condition |
94C_2min->(94C_30s->65C_30s,-1C/cyc->72C_30s)x10->(94C_30s->55C_30s->72C_30s)x35->72C_4min->10C
|
| polymerase |
Toyobo rTaq DNA polymerase
|
| Genebank No. |
|
| annotation |
weakly similar to OSJNBa0060B20.10 {Oryza sativa (japonica cultivar-group)}, partial ( 6%)
|
| similar marker |
ACM130
|
| product size in J |
240
|
| product size in Kaizuka-Wase |
|
| product size in Iki-White |
|
| product size in Tender Pole |
|
