| Crop | tomato |
|---|---|
| Population | GM_RIL |
| Linkage Group | GMRIL_02 |
| Marker Type | SSR |
| Map Position | 38.4 |
| Annotation | |
| Source Name | LeTC149677S |
| Source Type | cDNA |
| Source Sequence | attccattttctttcccttcttcagtatccttattttttt ctttcaaaaaaaaaatgaatccttctaccatagtcatgac aaaagagctctccgctggatcatcacgatccggcggtgaa cagttacaaAATAATAATcccgcaccgttgagtcggtacg agtcacaaaaaaggcgagactggaacacttttggtcagta cttgaaaaatcaaagaccacctgttccgttatcacagtgt aattgcaatcatgttctagaattccttcgataccttgacc agttcggtaagactaaggttcacttacacggatgcgtgtt tttcggacaacctgatccaccagcaccttgtacttgcccg ttaaggcaagcctggggtagccttgatgctttaataggga ggcttagagctgcgtacgaagagaacggagggtccccgga gaataatccgtttggaaatggcgcgattcgcctttattta AGAGAGgtaaaagagtgtcaagctaaggcaagagggattc cttacaagaagaaaaagaaaaggaagctTAATAATAAttc aattaagcccattggcgctggtgttggtgctagtgctgat caacacaaaaatttgatgcaagcaagctaaattataagga TATATAgggtggtcacatgaattggatgtatctgaagtag aTATATAgaaGATGATGATgcctgccacaagacaaatatg tacctcaatatactagctatctagctagttatgttttctt attaagtTATTATTATTATatttTATATATATATATATAT ATAcacatactgaagtcttagtctataataatttagttag ataatcagtttctttcaatcaaataactgtcttttggaaa tttgttaagggatttctcctccgttgtaaattaaattact tctcagctatttttataaatatactttgcatcattttcag caa |
| Developer | NIVTS |
| Document | Ohyama et al. (2009) Mol. Breed. (in press, DOI: 10.1007/s11032-009-9265-z) |
| motif | (tat)4attt(ta)10 |
|---|---|
| PCR product (bp) | 212 |
| fwd primer | |
| fwd primer seq. | ATGATgcctgccacaagacaaata |
| fwd postlabel primer | tme0103U |
| fwd postlabel primer seq | atgatgcctgccacaagacaaata |
| rev primer | |
| rev primer seq. | caacggaggagaaatcccttaaca |
| rev postlabel primer | tme0103L |
| rev postlabel primer seq | gtttcaacggaggagaaatcccttaaca |
| postlabel dye used | R6G-ddCTP |
| PCR condition | 94C_3min->(94C_30s->65C_60s,-1C/cyc->72C_60s)x10->(94C_30s->55C_60s->72C_60s)x30->75C_5min->10C |
| polymerase | Roche Taq |
| Line | allele-1 | allele-2 |
|---|---|---|
| A10 (PL11) | 221 | 221 |
| Ailsa Craig | 217 | 217 |
| Fixed Timely | 221 | 221 |
| LA716 (S.pennellii) | 213 | 213 |
| LA925 | 213 | 217 |
| M82 | 217 | 217 |
| MMF (Fixed Momotaro) | 221 | 221 |
| Micro-Tom | 217 | 217 |
| PL9 | 221 | 221 |
| Reika | 217 | 221 |
| Geronimo | 216 | 220 |
| Momotaro 8 | 220 | 220 |
| Population | Linkage Group | Position |
|---|---|---|
| GMF2 | GMF2_02 | 42.784 |
| GM_RIL | GMRIL_02 | 38.4 |
| DB | Program | Definition | E-Value |
|---|
| EST Name | Species | Strain |
|---|
