| Typing_Method |
Tm-Shift
|
| Marker_Class |
EST-Sl
|
| STS_primer_fwd |
CCGAAGCTGATGGAATTGACTATACTG
|
| STS_primer_rev |
TGAACCTCCAACTATCTCCCTCTCC
|
| amplicon_seq |
NNTCTCATTTCTTTTATAAATTTCCTGATTCGAGCAGCTCGAGTTGTTGGTAGCAACGCTTATAGATCTTGATGCAATGGATGGTAAAAGTAGTGTATCCTTGCTGGCCGAGTGTTCAAGTTCTCCTGATGTAAACACAAGGTTAGCTTTTTCACCCTCTTTTGACATTTCTATGAAATGAATTAGACCCCGTTGTACTCTATTACGCGCTATCATTTCTAATCTGTGCAATTAAACCTCACATTTTTCGTACTCGAGGCTCCCAAAATAGTGTTTCTGCCCCTTAAAATCAACTGCACTTCGTTTTGGATGATACTGAGGCATTAATTTGTTTGTCTGGTTTTGACTTGACACGCGTTTAAGAAAACAAAGAGAACTTTTGAATCTTGTGTCTTAAACTAAAGATACGTAGTAGAATGTACCAAAATGTCTTTTAATCTTGTGTTCTTAAGCATGCCACGTGAAACTTTTAACCCAAAGAGTTGCCAATAAAAAGGAAATAGACATTCTTTTTTAAACGGACTGAAAAGGAAAGTAAGACAAACAAATTGAAACGGATGTAGTTTAACTGTTATTTCCAGCTTAAATTCAACCTCAATTTTTATTAGTTAAGAAATTATTGAACTTAGGAGGCTCCTAAAGATTGAATCTGTATCATCTTGAGACGAAGATGAAGGCGTTTTACTATCCTCATTTGTTTTATTTGTCTTTTACTTGTTTTGATTGATACAGTATTAGTCATTTTAGATCTGTATGACTCGAACACCTGTAAATTTCATCGTGCAGGAAAGCATTAGCAAATGCACTTGCAGCTGCACCGTCCATGTGGACTTTAGGAAACGCTGGCATGGGAGCGTTCCAGGTAATTTGTATAGGAAACTGATGTCTTATCATACAATTTCCATCAACTACCCTTTCTCTTATTGAAGCTATTCAACACTGACGGAAGGCTTTGTTATG
|
| SNP_position |
245
|
| allele_1 |
T
|
| allele_2 |
A
|
| ASP_primer_S |
GCGGGCTTATTTTGGGAGCCTCGAGTACGAAAT
|
| ASP_primer_L |
GCGGGCAGGGCGGCTTATTTTGGGAGCCTCGAGTACGAAAA
|
| Common_primer |
CGCGCTATCATTTCTAATCTGTGCAAT
|
| enzyme |
Native Taq
|
| dye |
SYBR Green
|
