| Typing_Method |
Tm-Shift
|
| Marker_Class |
SOL
|
| STS_primer_fwd |
CAGCCTGGAGTCTATCCGAACATT
|
| STS_primer_rev |
CGGGAGCAAATAAGGGTTGTATCA
|
| amplicon_seq |
ATCCGAACATTGAGATGGCCGTCTCTGCTGCAAGGTTAGCATTTTNGTTTTAGCTTATTTTCTCTGTTATGATCTTCGTCTTACAAGCTAGCTTCATATATGGATGCTGTTCAGGCCTGGCGACACTATACTGATTGCTGCAGGAGGCAGCCATCATGTGTCAAATATTCAGATTGAAAAGCCACTTTGCTTGGTAACTTCAAAATGTTAACCTCAGATTACTTTATATACTTAAAGCGTACAAGATGTTGGGTCTTTTGATGTGTTTTGAATCTTCTGAGATTTCTGATAGTTATTTTAAAGCTATATATTTATGTGAAGTCACATTGTAGCTATTTCCACTCATATGATGCACTTGGGACGAAAATTTTCTTCCTTGCCATATAAGCATATGCCTTGCAAGAGTAAAATAAATGCATGCAAGTTATCTGAGACAGCACTAGCCTTTAGCTGCCTCAACTTCCTTTTTACAGATTGGTGCAGGTGAGAGTCCTGATGATACAACCCT
|
| SNP_position |
421
|
| allele_1 |
C
|
| allele_2 |
T
|
| ASP_primer_S |
GCGGGCCCTTGCAAGAGTAAAATAAATGCATGT
|
| ASP_primer_L |
GCGGGCAGGGCGGCCCTTGCAAGAGTAAAATAAATGCAGGC
|
| Common_primer |
TCTGTAAAAAGGAAGTTGAGGCAGCTAA
|
| enzyme |
Native Taq
|
| dye |
SYBR Green
|
