| Typing_Method |
Tm-Shift
|
| Marker_Class |
SOL
|
| STS_primer_fwd |
TTCTCAAGAATGAATGGGGAGTCA
|
| STS_primer_rev |
AGACATCCCACACCAACTTTGTCA
|
| amplicon_seq |
NNNNNNNNCGTGGCAGATAACTTATCTTATTTNATATTTTTATTTTATTCTTAGTAATTATTCTTGAAAATTACAAATAACTCAATTGATAGAGATGAGACGTAAACAGAGTAGATACTCAATTAAAAGTGATTATATCTTAACACATAAATAAGCACAAAATAGTTTAAACCATTTCTAATTAATATTTCTTAAGAATGTGTAAAAAGAAAACACAACAAATAATTTGAGACATAGCGAGTATCAATCATTTTTGCACATTATAGACAAACCAATTGATTGAGATGACACATAAAGAGAGCAAATATTCAATAGAGGGTAATTACTCATATTTTAAGACATAAATAAGGATACAATAATCAAAAACTTCTCCTAAATTATACTTCCTCAGTCCACAATTATTTGGCAGATATATTAAAAATAGATGTCCAAGATAAATTGTCAATTTAAATAATCAAGAAACAATTAGTCATTTTTTTTCAATTTTACCCTTAATAATAGGGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATATTAGTCAAATTACACATTATATTAAATTTTACTTGAGGAATATGCATAACCTTAATATGACAAGCAATTATAAACGAAAAGAATATTTTTTAAGAAGCGTGTAAAAGCAAATTACTGACACATAGTTTTTTTGAGACGGATGGAGGGAATGTGTATGTATTAAAGTTAGCATTTAAAGCTAACCTCCAATGTTGGTGAACTAATAATAGAGTCAATAACTATACAATNNATATACACCATGGCCCTACTACAAACTTGTTGCCATTTAATTTATCAATGTTACTAACATTTATGTACTTTTATTTT
|
| SNP_position |
691
|
| allele_1 |
G
|
| allele_2 |
A
|
| ASP_primer_S |
GCGGGCACTGACACATAGTTTTTTTGAGACA
|
| ASP_primer_L |
GCGGGCAGGGCGGCACTGACACATAGTTTTTTTGAGCCG
|
| Common_primer |
TGCTAACTTTAATACATACACATTCCCTCC
|
| enzyme |
Stoffel frag.
|
| dye |
EvaGreen
|
