| Typing_Method |
Tm-Shift
|
| Marker_Class |
SOL
|
| STS_primer_fwd |
ATAAGAAACGGTTCAGCAGCTTGG
|
| STS_primer_rev |
TTGACATATTTCACCTTCCCAGCA
|
| amplicon_seq |
ATGGTTTGTCAGTCCTGCCATTGCATTCTATTTTTCTTTTAGTTTGTGTCAAATGACCACAAGTTGCAGCAGCTATGGTTACATCACACTCTTCTTTCCTGTGTTCTTTTGCTTTCTTATTCACAGTTTGAACATGATACTGTTTTGATACCAAAGGAGACTTCATGGTTTGGATACTATCCAGATGGGGCCTTTGAACCTATTTTGCCAGCACAGAAGGTATGATGTGGAATCTAGTTAAGAGAAATGTTCAATTTATATTATTTTTGCTGCCATTTCAGCAAACAAGACACCCTCTCAATTTTTTTTTCATATGTATGATATCCAAAAAAAACCAAATGCTTGCATTGTGTATCTGTATATATAACACTTCCTTGACAAATGCACTATTTTCATACTGAATTTCCTGTTCCTTCCTCAACAAGTTCAAATGAGTTGTCAATATCCTTCATTCCACGAAAGAGCGAGACCTTTTCCTTGTTGACGATGTTCTTCACCAGTACTGTTTTCGCTGTGGTTTNGACATGCTTCAGAGAAAAATGTTAATATGANAACCAAATGTATGGGTTACTTAAATGTTCCAATGATGGCAACATATCTACTTCTGGATTACACCGCCACCTCCTTGCTTCTCACTGTTCTCCCAAGTCTTCAACATAAACACTATCACCTTACTTGGTATACTTGCGTCGTATTCTGACAATTACATACCTCCAACCTGCAATTAGTCATATCAGTAACCATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCAGGGCCAAGCTTTAAGATCTAGTTAATCTTGGCTCAACNTGAATATGCTATGCATGGCTTTGACTTNGAGATTCAGCTTCATTGATACTTAAAATATGTTTTATTGTGATTCCAGAACAAATATGGAAAGCTCGAGATCAATATGTTAATAATTCAAAGATAAAAAATTTCATGACCTTTCTTCTCCTCTATATTGTTTTAATCAAGTCTTTCTTCCATTTATGTCTTGCATTTGCATATTTTACACTAAATGCAAGTAAACCACTTGAAGCTACCAGAGNNAACATTAACTTATTCAAGTAGGCTCTATTAGACGTACAAAAACCTCACTGTTTCAATTCAACATGTATCAAACTAGAGTCAATTCATTGATTTTGATCTAGAGCTAGGCCTTTATAGGTTCAATCATGTTGGCAGGGCAGCGTCAGTCCAAGGAGTTGCCTCTAGAGTTTCTATTCTGTAATATTAGACATCCTGTTTTCTTTATGTTTTAATGATTAACGGAAATAATTGGTTCAACTTCCTGATGGAGTCTGCTCTTGTGTTTCTTGCTAATCAACTGTGACCAAGTGTTTCATCTCTCCTGCACCCCATTCTTTTTCCCATAGTTCTATCCATACATTTTACCCTGTACCACATGATCCTATTAGTTAAGCACATTTCAGGTTCGCGAAAACTATGCTTGACAATATTTGAAAAGATACTTTGTTTTCCTGTACATTTGTGCTTCAAGTTCAACCAAGTGAGATTTTTCCTTTTGCTTGGCAGACTCAGCTGTATACTGAGGATGGATCG
|
| SNP_position |
627
|
| allele_1 |
T
|
| allele_2 |
G
|
| ASP_primer_S |
GCGGGCAGGGCGGCGAAGACTTGGGAGAACAGTGAGAAGCC
|
| ASP_primer_L |
GCGGGCAGGGCGGCGAAGACTTGGGAGAACAGTGAGAACCC
|
| Common_primer |
TCTACTTCTGGATTACACCGCCACCTC
|
| enzyme |
Native Taq
|
| dye |
SYBR Green
|
