| Typing_Method |
Tm-Shift
|
| Marker_Class |
EST-Sl
|
| STS_primer_fwd |
CGTGTGAGCTTGTTCCAAGAGAAA
|
| STS_primer_rev |
GATCAGGCAATGAATGCTCGATAA
|
| amplicon_seq |
NNNNNNNNNNNNCCAAGAGAAATTGCAGGAATTGATGGACTTGAATAAAGGTAAGTTTGGATCTTTCTATAAATTTCTGTGTGGTAGAGAAATAGATAAAGAGTTGTGCCAGAAAAGTTATGAAGTAAATGATTCGTTTAAAGTAATAACAGTGTGTCCACTTTTGATACTATTGTTTCTGATCAGTAAAAAAGAGAAAAGTATCATGCTTGCCTCCCATAACATGATGAAGAATGGGTGTGTGCTGGAGTGGATATTCAAATGATTTCTGTTTGTTCAGCATATAATAGTTATTGTTGAAGATGTGTTATTTAGAAAGTTTGATTTGTTTCCATATGACGTAACAAGGACAAATGTTGAATACTGACTATTCTCAATTTGTCCTATAAGCGCCATTGCGACCATCAGCAAGGATAAATCCGCAAGGTGCTGCTCGATTTATCGAGNNNNN
|
| SNP_position |
351
|
| allele_1 |
C
|
| allele_2 |
A
|
| ASP_primer_S |
GCGGGCTTTGTTTCCATATGACGTAACAAGGAA
|
| ASP_primer_L |
GCGGGCAGGGCGGCTTTGTTTCCATATGACGTAACAAGNAC
|
| Common_primer |
GCAATGGCGCTTATAGGACAAATTGAG
|
| enzyme |
Native Taq
|
| dye |
SYBR Green
|
