| Typing_Method |
Tm-Shift
|
| Marker_Class |
SOL
|
| STS_primer_fwd |
TTACAAGAACGGACCAGTTGAGGT
|
| STS_primer_rev |
GATGAGCTTAACAGCATGGCCTC
|
| amplicon_seq |
NAGCNNNNNACAAAACAGTAACATAAGTTACTTGGTTATACCGGATTTTGCTCCCTACCACTCAAGAGTTTACCAGCTCGCAACCTGGGAGTATATCGAAGGCCATGCTGGTAGGCTCATCANACGAATCATCTGATGANNNNNNNNNNACTTTAGTTAAATATATTACAAATATCCTTTTCTAATTTTTCAAATGTGATTTCCTTACAAATCATATNNNNGGACAATAATAATGCTTTGCTGAATCAAAAAGCAAAATATAATTGAAATCACTTTCTTTTGATGGTTAACTGTCAAATAATTGTTGATAANNNNTTCAACAGAGTTCAAAATATTTGTTGGTCACCAACTCTACCTTTCTGAATTCTAGTGTTACCATCTACTATGTTTGTGGAAATATTGTGTCAATTAGTGATCTGTAGAGGTTTATGTTCCAGGATAATTATGTCCATGACAATGAAGTACCAGTTAGGAAGTCCAATGATCTGCTTAAATGCATCTAGAGGTCATTGTTTANNNAATGCACTAATTTCTCACCTCTCTTCTGGTGAAANTTGAANNATTNNTGCCGACTTGGATTAGTTTCTTTACTNNNNCTTTCTNNNGTNNNNNNNNNTATCTTCTCCTATTTTTACTGGTGACCTTCCTATACTGAATGACANAACTGTTTCTCGAGCACAGGATNNNTTCGCTCACTACAAGTCTGNNNNNGTGTTTACAGCACATAAATGGTGANNNNNNNNGGAAATNNN
|
| SNP_position |
473
|
| allele_1 |
G
|
| allele_2 |
A
|
| ASP_primer_S |
GCGGGCAGGGCGGCATGCATTTAAGCAGATCATTGGACTTC
|
| ASP_primer_L |
GCGGGCAGGGCGGCATGCATTTAAGCAGATCATTGGACCTC
|
| Common_primer |
TCCAGGATAATTATGTCCATGACAATG
|
| enzyme |
Native Taq
|
| dye |
SYBR Green
|
