+Marker Data: emx10304

Crop eggplant
Population LWAE2012
Linkage Group E_01
Marker Type SSR
Map Position 25.518
Annotation
Source Name Sme2.5_02438.1
Source Type genomic
Source Sequence TATTTATGACTTTCTTCGTCTCAAATTAATTAAATTTTTG AGATTTTTTTCAATGTTTAAAATAAATGAATTCTTCAAAG TTTAAAAAGAATATTTGAAACTTTTTTCATATTTGCCCTT TCATTTATTGAAGTTTTATATTTTCTAGGAGATAAATTAC ACTACATATTTATTATTTCATAAAGAACAATAATGGAAAG TATGATATAAATTTTATTTTTAAATACTTTTCTTAATATG TGTATGTTACTTGATAAATTTAATTAATATGAAACGGAGA GTGTAATAAGGAGAATCCAAAGTTCAAAGCACGTTATCTA TAAACAATATATTATTAGTAAGGAAATGAATGATCACATG ATATCTGGTTAACAAATTAAATAAAATTATGTATATATTC AGGTTGGAATTTGTTTTCTTTTTACACTTACAATTTTTTA AATATTTTATATCACATTCGAATCTAGTACTAATATCTAG ATTTTGACTCTTGTCAATAAATATATATATATATATATAT ATATATATATATATATATATATATATATATATATATNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNAGATATATATATATATATATATA TATATATTATGTGTTATGTTACATAATAAGAAATTGATCG ACATGAAATTTGATGAAATGATTTGAGATATATTTTTCTT AAATAAAAAATTTCGAGTTCAAGTCTCTAGACATCTCAAT GAATCATAATGCACCATAACATATAATAATATATCTAGTG AATTTTTACAAGTGAGATCTGAGAAGATCGATCGAGTGTA AGCAAACTTTATTATTATCTCATGGAGATAGAGACAGGGG CAGAGTCACTCTTAGCCGAGGGGAGCGACACCCCTTTTGT CGAAAAAGTATATTGCATAATATATATGTCAAAAGTTAAT TTAATAAATATTTATACAAGTATTGACATCTCTTTGATAC AACCTAAATATTTAATTTAGTGGTTAAGAGGTTGCAAAAA TTGTTTGAGGTCATTTG
Developer NIVTS
Document
motif (AT)22
PCR product (bp) 378
fwd primer emx10304/F
fwd primer seq. TCAGGTTGGAATTTGTTTTCTTT
rev primer emx10304/R
rev primer seq. TGGTGCATTATGATTCATTGAGA
fluor. primer emx10304F_NED_s01
dye NED
fluor. Primer seq. NED-ACAGGTTGGAATTTGTTTTCTTT
PCR condition 94C_3min->(94C_30s->65C_60s,-1C/cyc->72C_60s)x10->(94C_30s->55C_60s->72C_60s)x30->75C_5min->10C
polymerase Roche Taq
Line allele-1 allele-2
Population Linkage Group Position
LWAE2012 E_01 25.518
N28F2 E_01 143.5
DB Program Definition E-Value
EST Name Species Strain