| Crop | tomato |
|---|---|
| Population | EXP2k_tm_081031 |
| Linkage Group | T11 |
| Marker Type | SSR |
| Map Position | 61.85 |
| Annotation | |
| Source Name | LeTC152176S |
| Source Type | cDNA |
| Source Sequence | actacccaactcaccttatacaagcacccaatatacaaaa gggggcttactttacaagctacctgaagaaaaccttcagt atgtgacatccattacttctctgctcaccacttatgccaa atacatggcaactaaaaagcatactttcaactgtggaagc cttgtcgttacagaaaagacaattagaaactttgcaaaaa gacaggtggactatattttgggaaataatccaatgaaaat gtcatacatggctgggtatgggagtaactacccgcgaaga attcaccatagaggatcatctttaccttctttggcaatgc atccacaatcatttggttgtgaagctggatttcaaccatt ttactacacagcaaatcctaaccctaacatattagttgga gctattatcggaggtccaaatcaaaatgatttcttcccag atgaacgtactgattatagtcattcggagccagctactta cattaatgctgctattgttggacctttagcatattttgat agttccaaacattaacggtttatctaattgtaattgtaaA TATATcccttcatcaccaaaacttggtatagggaTATATA cttacttcttaaTATATATATATATATATATATAtgttca acgaatgtttcttccccatctcaaacttaattatgccaaa cc |
| Developer | NIVTS |
| Document | Ohyama et al. (2009) Mol. Breed. (in press, DOI: 10.1007/s11032-009-9265-z) |
| motif | (ta)3cttacttcttaa(ta)11 |
|---|---|
| PCR product (bp) | 265 |
| fwd primer | |
| fwd primer seq. | tggagctattatcggaggtccaaa |
| fwd postlabel primer | tme0119U |
| fwd postlabel primer seq | aggagctattatcggaggtccaaa |
| rev primer | |
| rev primer seq. | agatggggaagaaacattcgttga |
| rev postlabel primer | tme0119L |
| rev postlabel primer seq | gtttagatggggaagaaacattcgttga |
| postlabel dye used | R6G-ddCTP |
| PCR condition | 94C_3min->(94C_30s->65C_60s,-1C/cyc->72C_60s)x10->(94C_30s->55C_60s->72C_60s)x30->75C_5min->10C |
| polymerase | Roche Taq |
| Line | allele-1 | allele-2 |
|---|---|---|
| A10 (PL11) | 269 | 269 |
| Ailsa Craig | 269 | 269 |
| Fixed Timely | 269 | 269 |
| LA716 (S.pennellii) | 275 | 275 |
| LA925 | 267 | 267 |
| M82 | 269 | 269 |
| MMF (Fixed Momotaro) | 269 | 269 |
| Micro-Tom | 269 | 269 |
| PL9 | 269 | 269 |
| Reika | 269 | 269 |
| Population | Linkage Group | Position |
|---|---|---|
| EXP2k_tm_081031 | T11 | 61.85 |
| DB | Program | Definition | E-Value |
|---|
| EST Name | Species | Strain |
|---|
