| Crop | tomato |
|---|---|
| Population | EXP2k_tm_081031 |
| Linkage Group | T08 |
| Marker Type | SSR |
| Map Position | 97.21 |
| Annotation | |
| Source Name | LEFL2020D05S |
| Source Type | cDNA |
| Source Sequence | tggatttcgtcgatttccataatctcttgtctgTATATAT ATATATATATATATATAcgtacatcatcgctgttcttact ctgaaagtttcacaaactgatagattttgtattgnaattt tgttgaattcaattgaattgtgtattggaaggaatcnaat ttgaatcgagaanatggattcttcnatacccacttctcan tcggaagaacggaagccgganganccagaaaccaaanaaa tggtgcacaaaacanaggttattcnnttcttgggccgaac aacaccaatcattcttcanaacgatnatggaccttgtcca cttcttgctatctgnaatgnactattgttgaanaacaact tgagcttgagccctgatattcctgangtttcacanganaa attgcttTCTCTCgttgctgagcggctgatanattcaaac agnantgtcaatgacaaanatgctggatatgttgaaaatc ancaacagaatattgnngatgcnnttgatttgcttcctan acttgcnactggtattgatgtaaatataanattcnngaga attgatgactttganttcnctcgtgnntgngcaatatttg atcttnngnatatnccac |
| Developer | NIVTS |
| Document | Ohyama et al. (2009) Mol. Breed. (in press, DOI: 10.1007/s11032-009-9265-z) |
| motif | (ta)12 |
|---|---|
| PCR product (bp) | 291 |
| fwd primer | |
| fwd primer seq. | cgtcgatttccataatctcttgtctg |
| fwd postlabel primer | tme0100U |
| fwd postlabel primer seq | agtcgatttccataatctcttgtctg |
| rev primer | |
| rev primer seq. | tgaagaatgattggtgttgttcgg |
| rev postlabel primer | tme0100L |
| rev postlabel primer seq | gtttgaagaatgattggtgttgttcgg |
| postlabel dye used | R6G-ddCTP |
| PCR condition | 94C_3min->(94C_30s->65C_60s,-1C/cyc->72C_60s)x10->(94C_30s->55C_60s->72C_60s)x30->75C_5min->10C |
| polymerase | Roche Taq |
| Line | allele-1 | allele-2 |
|---|---|---|
| A10 (PL11) | 299 | 299 |
| Ailsa Craig | 299 | 299 |
| Fixed Timely | 293 | 293 |
| LA716 (S.pennellii) | 281 | 281 |
| LA925 | 289 | 289 |
| M82 | 295 | 299 |
| MMF (Fixed Momotaro) | 289 | 293 |
| Micro-Tom | 293 | 293 |
| PL9 | 299 | 299 |
| Reika | 293 | 299 |
| Population | Linkage Group | Position |
|---|---|---|
| EXP2k_tm_081031 | T08 | 97.21 |
| DB | Program | Definition | E-Value |
|---|
| EST Name | Species | Strain |
|---|
