+Marker Data: tme0075

Crop tomato
Population EXP2k_tm_081031
Linkage Group T08
Marker Type SSR
Map Position 100.01
Annotation
Source Name LeTC144862S
Source Type cDNA
Source Sequence caacttttgacatttcttgataaattggctttgaattttt cattatgagtagtaaagagttgttgagaatgaaaactaaa aaggcaaatgggaatggagttTCATCATCAggatgggaaa tgaggccaggaggaatgttagtacaaaagagaagttctga tcataattcaaatcaaagttcaaatattgttgttccaatt attagactcaaagttaaatatggttcatcttatcatgaag tcaaaattagctcacaagcaacttttggggagttgaagaa aatgttggcagggccaactggattacatactgaagatcag aaaatattttacaaggaaaaagaaaaagattctagaaatt ttcttgatgtttctggtataaaagatggatcaaagcttgt actaattgaagatgaaataagcagagaaaaaaggtatctt gaatcaagaagaaatgcaaaaatggaaaatgcatcaaaag aaatcacatcaattagacttgaaattgataaactagctaa acaggttgcaaatgttgaaatggacatttatggtggcaaa aaagtaacagagactttgttattgagtttgattgaaatgt taatgacacaattgattaagttggatggaattactgctga tggtgatcttaaattacagagaagaatgcaggtgaaaagg gtgcaaaaatacatagagactctagacatgttgaaaataa gaaattcaacacttggaaatgacaatgccaaagtgtcaat gaagcacaaaaatagaatattcacaggacaaatggcaaaa tcaatttattatcaacaagaacaaagaaagatgggaaatt ttgctgatgagaaaagcccagggccagttgtagtgacaac aaaatgggaaactttctaagaattgattaataAATAATAA TtaaaaaaagcctaaggaatattagTGTGTGctttgaaaa TATATATATATATATATATATATATATATATAattcagtg gctttggtggaaagggtataaagttagagggtacataaat aaattttggttttgtgaattcatttgccttcatttctttt tcctatttagatttttgaccccaaatgtttttggggttga aaaaataatgtggtgatatgttatgtagcctttgaattcc aagttgtataaatgtgTATATATAatgccagcatctggtg aattg
Developer NIVTS
Document Ohyama et al. (2009) Mol. Breed. (in press, DOI: 10.1007/s11032-009-9265-z)
motif (aat)3taaaaaaagcctaaggaatattag(tg)3ctttgaaaa(ta)16
PCR product (bp) 185
fwd primer
fwd primer seq. attttgctgatgagaaaagcccag
fwd postlabel primer tme0075U
fwd postlabel primer seq attttgctgatgagaaaagcccag
rev primer
rev primer seq. tttataccctttccaccaaagcca
rev postlabel primer tme0075L
rev postlabel primer seq gtttataccctttccaccaaagcca
postlabel dye used R6G-ddCTP
PCR condition 94C_3min->(94C_30s->65C_60s,-1C/cyc->72C_60s)x10->(94C_30s->55C_60s->72C_60s)x30->75C_5min->10C
polymerase Roche Taq
Line allele-1 allele-2
A10 (PL11) 186 186
Ailsa Craig 186 186
Fixed Timely 186 186
LA716 (S.pennellii) 150 150
LA925 176 176
M82 176 176
MMF (Fixed Momotaro) 176 176
Micro-Tom 186 186
PL9 186 186
Reika 186 186
Population Linkage Group Position
EXP2k_tm_081031 T08 100.01
DB Program Definition E-Value
EST Name Species Strain