+Marker Data: tme0052

Crop tomato
Population EXP2k_tm_081031
Linkage Group T08
Marker Type SSR
Map Position 1.48
Annotation
Source Name LeTC142310S
Source Type cDNA
Source Sequence atggcaaatattctaccaatttcaataaattaccacaagc caaaactagtagttccatcaagtgtaacatctcatgagac aaaacgtctttctgaaatagatgatcaagggtttattcga ctccaaatccccatactaatgttttacaaatacaattctt caatgaaaggtaaagatctagcaaaaattatcaaagatgg attatctaaaacacttgtgttttactatccattagctggt agactcattgaagggcctaataaaaagcttatggtaaatt gcaatggtgaaggagtcttgtttatcgaaggtgatgctaa tatagagcttgaaaaattaggtgaatctattaagccacca tgtccatacttggatttactacttcataatgttcatggtt ctgatggaattattggttctcctcttttgttaattcaggt ggagacgcggatattttgCTCTCTtttaaggagatgcaag ctcactgcgatataatctacaccgattcagcagtatcgct cttgacttgtctcctccagaatacaacatcccaacgatgt gtacaactcaagcaacgtcggattaattgaaaagtcaaaa gtttcacgaaaagaacgtcttccttaaacatatacacagt gaatatagatagttgaatacttcggaatattttctttgta gtctcaatTCTCTCtttcactacactaacctcaatataaa tttacattaggtctcatcttcttgagatacttcacaaaga cagaaacaccaatattttatagctgaagaaatcttccata taattaaggtggaatgaggggtagtaatgtcgatcggtag ataaatgtgatagatattacatgagttccaagaacaaata gtcatcttctaccatacgttataccactaactgatgcact taatattttaataatcaaatgaatatagaccaagcattct accttctttattcaagtTATATAttaatcatactctttct ataaatggttaaattcttagactaaTATATATATATATAT ATAcctctttgaataggtgactcgttttacttgtggtgga tttgctgttggatttagatttaatCACACAatgatggatg cttatggcttcaaaatgtttctaaatgcgttaagtgaatt aattcaaggagcttcaacaccttctatattgcctgtatgg gaaagacatctcctaagtgctagatcatcaccaagtatta catgtattcatcatgagtttgatgaggaaattgaaatcaa aaattgcgtgggaatctatggaagataagttgatacaaca atcatttttctttgggaaatgaggagatggaagtcatt
Developer NIVTS
Document Ohyama et al. (2009) Mol. Breed. (in press, DOI: 10.1007/s11032-009-9265-z)
motif (ta)9
PCR product (bp) 277
fwd primer
fwd primer seq. tgaggggtagtaatgtcgatcggt
fwd postlabel primer tme0052U
fwd postlabel primer seq agaggggtagtaatgtcgatcggt
rev primer
rev primer seq. ccaacagcaaatccaccacaagta
rev postlabel primer tme0052L
rev postlabel primer seq gtttccaacagcaaatccaccacaagta
postlabel dye used R6G-ddCTP
PCR condition 94C_3min->(94C_30s->65C_60s,-1C/cyc->72C_60s)x10->(94C_30s->55C_60s->72C_60s)x30->75C_5min->10C
polymerase Roche Taq
Line allele-1 allele-2
A10 (PL11) 281 281
Ailsa Craig 281 281
Fixed Timely 281 281
LA716 (S.pennellii) 277 277
LA925 279 279
M82 281 281
MMF (Fixed Momotaro) 279 281
Micro-Tom 315 315
PL9 281 281
Reika 281 281
Population Linkage Group Position
EXP2k_tm_081031 T08 1.48
DB Program Definition E-Value
EST Name Species Strain