| motif |
(ta)3ttactttgtgtcgagactgttc(ac)14a(ta)3
|
| PCR product (bp) |
202
|
| fwd primer |
AFA07G10-U
|
| fwd primer seq. |
atcgctgtgttgtctgattctgtg
|
| rev primer |
AFA07G10-L
|
| rev primer seq. |
gtttcaaatggttaggagcaaccatcc
|
| fluor. primer |
AFA07G10-F(VIC)
|
| dye |
VIC
|
| fluor. Primer seq. |
VIC-atcgctgtgttgtctgattctgtg
|
| PCR condition |
94C_2min->(94C_30s->65C_30s,-1C/cyc->72C_30s)x10->(94C_30s->55C_30s->72C_30s)x35->72C_4min->10C
|
| polymerase |
Toyobo rTaq DNA polymerase
|
| Genebank No. |
AB499388
|
| annotation |
|
| similar marker |
|
| product size in J |
190+220
|
| product size in Kaizuka-Wase |
180
|
| product size in Iki-White |
190
|
| product size in Tender Pole |
190
|
