+Marker Data: AFAT05G01

Crop bunching_onion
Population DJF2
Linkage Group Chr8
Marker Type SSR
Map Position 204.2
Annotation
Source Name AB499457
Source Type genomic
Source Sequence
Developer NIVTS
Document Tsukazaki et al. (2007) Euphytica 157:83-94
motif (tg)3ctatgtgcacgtg(ta)4attgc(gt)3a(ta)3ag(tg)4cgttc(gt)3atatca(gt)3(at)3gacagatt(tg)3c(gt)3agtatacctgtatgttag(tg)4cgtgagtgtaattt(tg)5ttgc(gt)3atagg(ta)4aa(tg)8
PCR product (bp) 300
fwd primer AFAT05G01-U
fwd primer seq. agtgtgttgcagtgtttgtgtatg
rev primer AFAT05G01-L
rev primer seq. gtttccactacaccgcacatccctatatc
fluor. primer AFAT05G01-F(FAM)
dye FAM
fluor. Primer seq. FAM-agtgtgttgcagtgtttgtgtatg
PCR condition 94C_2min->(94C_30s->65C_30s,-1C/cyc->72C_30s)x10->(94C_30s->55C_30s->72C_30s)x35->72C_4min->10C
polymerase Toyobo rTaq DNA polymerase
Genebank No. AB499457
annotation
similar marker
product size in J 300
product size in Kaizuka-Wase
product size in Iki-White 310
product size in Tender Pole 310
Line allele-1 allele-2
C1s-11s-25s-7 308 308
Cho1s-1s-2s-2s-8s 308 308
D1s-15s-10s-8 294 294
F1s-7s-17s-3s-1 308 308
J1s-14s-23s-9 308 308
K1s-5s-2s-2s-4s-5 308 308
Chiang_mai
Kaizuka_Wase
Population Linkage Group Position
DJF2 Chr8 204.2
DB Program Definition E-Value
EST Name Species Strain