+Marker Data: AFS140_2

Crop bunching_onion
Population DJF2
Linkage Group Chr7b
Marker Type SSR
Map Position 77.7
Annotation
Source Name AB499334
Source Type genomic
Source Sequence
Developer NIVTS
Document Ohara et al. (2005) Euphytica 144:255-263
motif (ta)3ca(ac)12a(ta)5tg(ca)3
PCR product (bp) 213
fwd primer AFS140_2-F
fwd primer seq. cgtagcggatggtcacaaga
rev primer AFS140_2-R
rev primer seq. tggacctggaggcctagact
fluor. primer AFS140-2-F(NED)
dye NED
fluor. Primer seq. NED-cgtagcggatggtcacaaga
PCR condition 94C_2min->(94C_30s->60C_30s,-1C/cyc->72C_30s)x10->(94C_30s->50C_30s->72C_30s)x35->72C_4min->10C
polymerase Toyobo rTaq DNA polymerase
Genebank No. AB499334
annotation
similar marker
product size in J 200
product size in Kaizuka-Wase
product size in Iki-White 200
product size in Tender Pole 200
Line allele-1 allele-2
C1s-11s-25s-7 209 225
Cho1s-1s-2s-2s-8s
D1s-15s-10s-8 219 219
F1s-7s-17s-3s-1 207 207
J1s-14s-23s-9 199 199
K1s-5s-2s-2s-4s-5 217 217
Chiang_mai
Kaizuka_Wase
Population Linkage Group Position
DJF2 Chr7b 77.7
FA 7c 14.1
P1 J09 7.0
P2 D09 0.0
SaT03 7b 26.6
DB Program Definition E-Value
EST Name Species Strain