+Marker Data: AFRA04B10

Crop bunching_onion
Population DJF2
Linkage Group Chr6b
Marker Type SSR
Map Position 33.1
Annotation
Source Name AB499515
Source Type genomic
Source Sequence
Developer NIVTS
Document Tsukazaki et al. (2007) Euphytica 157:83-94
motif (tg)5cagttgta(tg)8cgcgtgcatgcgtgcgtgtgcatgc(gt)3atgtgctac(tg)3ca(tg)4cagttgtgta(tg)5catagttgta(tg)7
PCR product (bp) 292
fwd primer AFRA04B10-U
fwd primer seq. atgagtgtgcagttttgtgagggt
rev primer AFRA04B10-L
rev primer seq. gtttgcgcaggtccatctgtaaaaa
fluor. primer AFRA04B10-F(NED)
dye NED
fluor. Primer seq. NED-atgagtgtgcagttttgtgagggt
PCR condition 94C_2min->(94C_30s->65C_30s,-1C/cyc->72C_30s)x10->(94C_30s->55C_30s->72C_30s)x35->72C_4min->10C
polymerase Toyobo rTaq DNA polymerase
Genebank No. AB499515
annotation
similar marker
product size in J 300
product size in Kaizuka-Wase
product size in Iki-White
product size in Tender Pole
Line allele-1 allele-2
C1s-11s-25s-7 298 298
Cho1s-1s-2s-2s-8s 298 298
D1s-15s-10s-8 284 284
F1s-7s-17s-3s-1 298 298
J1s-14s-23s-9 296 296
K1s-5s-2s-2s-4s-5 296 296
Chiang_mai
Kaizuka_Wase
Population Linkage Group Position
DJF2 Chr6b 33.1
KiCF2 6b 10.1
SaT03 6b 5.1
DB Program Definition E-Value
EST Name Species Strain