+Marker Data: ACE010

Crop bunching_onion
Population DJF2
Linkage Group Chr5
Marker Type SSR
Map Position 17.7
Annotation
Source Name CF436768
Source Type onion_EST
Source Sequence
Developer NIVTS
Document Tsukazaki et al. (2008) Theor Appl Genet 117:1213-1223
motif (ac)8g(cg)5tgc(gt)4
PCR product (bp) 180
fwd primer ACE010-F
fwd primer seq. atgtaccacatggatgaaaaacaca
rev primer ACE010-R
rev primer seq. ggtagctgaagcaaatcaagcaa
fluor. primer ACE010-F(PET)
dye PET
fluor. Primer seq. PET-atgtaccacatggatgaaaaacaca
PCR condition 94C_2min->(94C_30s->65C_30s,-1C/cyc->72C_30s)x10->(94C_30s->55C_30s->72C_30s)x35->72C_4min->10C
polymerase Toyobo rTaq DNA polymerase
Genebank No. CF436768
annotation
similar marker ACM133
product size in J 160
product size in Kaizuka-Wase 190
product size in Iki-White 180
product size in Tender Pole
Line allele-1 allele-2
C1s-11s-25s-7 167 167
Cho1s-1s-2s-2s-8s 167 167
D1s-15s-10s-8 167 167
F1s-7s-17s-3s-1 169 169
J1s-14s-23s-9 169 169
K1s-5s-2s-2s-4s-5 167 167
Chiang_mai 190 190
Kaizuka_Wase
Population Linkage Group Position
DJF2 Chr5 17.7
FA 5a-2 21.3
DB Program Definition E-Value
EST Name Species Strain