| motif |
(cg)4c(ac)4(tc)3t(ac)4gtgca(tg)3
|
| PCR product (bp) |
250
|
| fwd primer |
ACE039-F
|
| fwd primer seq. |
atggtcgtccatttcttattgaagt
|
| rev primer |
ACE039-R
|
| rev primer seq. |
ttgatgtatgaggcagcattctactg
|
| fluor. primer |
ACE039-F(PET)
|
| dye |
PET
|
| fluor. Primer seq. |
PET-atggtcgtccatttcttattgaagt
|
| PCR condition |
94C_2min->(94C_30s->65C_30s,-1C/cyc->72C_30s)x10->(94C_30s->55C_30s->72C_30s)x35->72C_4min->10C
|
| polymerase |
Toyobo rTaq DNA polymerase
|
| Genebank No. |
CF442638
|
| annotation |
similar to GP|8778607|gb|AAF79615.1|AC027665_16|AC027665 F5M15.23 {Arabidopsis thaliana}, partial (7%)
|
| similar marker |
ACM306
|
| product size in J |
260
|
| product size in Kaizuka-Wase |
300
|
| product size in Iki-White |
250
|
| product size in Tender Pole |
|
