+Marker Data: AFA07A10

Crop bunching_onion
Population DJF2
Linkage Group Chr4a
Marker Type SSR
Map Position 20.8
Annotation
Source Name AB499382
Source Type genomic
Source Sequence
Developer NIVTS
Document Tsukazaki et al. (2007) Euphytica 157:83-94
motif (ac)11a(ta)3tgtatg(ta)7
PCR product (bp) 237
fwd primer AFA07A10-U
fwd primer seq. actactgggatgaatgcccaaaga
rev primer AFA07A10-L
rev primer seq. gtttccagttgcacatgacttttg
fluor. primer AFA07A10-F(PET)
dye PET
fluor. Primer seq. PET-actactgggatgaatgcccaaaga
PCR condition 94C_2min->(94C_30s->65C_30s,-1C/cyc->72C_30s)x10->(94C_30s->55C_30s->72C_30s)x35->72C_4min->10C
polymerase Toyobo rTaq DNA polymerase
Genebank No. AB499382
annotation
similar marker
product size in J 200+210
product size in Kaizuka-Wase 200+210
product size in Iki-White
product size in Tender Pole
Line allele-1 allele-2
C1s-11s-25s-7
Cho1s-1s-2s-2s-8s 228 228
D1s-15s-10s-8 228 228
F1s-7s-17s-3s-1 230 236
J1s-14s-23s-9 238 238
K1s-5s-2s-2s-4s-5 240 240
Chiang_mai
Kaizuka_Wase
Population Linkage Group Position
DJF2 Chr4a 20.8
KiCF2 4a 174.7
DB Program Definition E-Value
EST Name Species Strain