| motif |
(aca)5
|
| PCR product (bp) |
200
|
| fwd primer |
ACM005-F
|
| fwd primer seq. |
cgcttcagcagtgagttgtt
|
| rev primer |
ACM005-R
|
| rev primer seq. |
tgttgtccgatacagagttgct
|
| fluor. primer |
|
| dye |
|
| fluor. Primer seq. |
|
| PCR condition |
94C_2min->(94C_30s->65C_30s,-1C/cyc->72C_30s)x10->(94C_30s->55C_30s->72C_30s)x35->72C_4min->10C
|
| polymerase |
Toyobo rTaq DNA polymerase
|
| Genebank No. |
BI095610
|
| annotation |
weakly similar to GP|4336436|gb|AAD17805.1||AF092432 protein phosphatase type 2C {Lotus japonicus}, partial (33%)
|
| similar marker |
JR853133
|
| product size in J |
200
|
| product size in Kaizuka-Wase |
200
|
| product size in Iki-White |
200
|
| product size in Tender Pole |
200
|
