| motif |
(tct)3tcg(tct)6(ct)3c
|
| PCR product (bp) |
182
|
| fwd primer |
AFC03F10-U
|
| fwd primer seq. |
agttcttgatcgcttagagggctg
|
| rev primer |
AFC03F10-L
|
| rev primer seq. |
gtttgaaggcacaatgtatgcttcgcta
|
| fluor. primer |
|
| dye |
|
| fluor. Primer seq. |
|
| PCR condition |
94C_2min->(94C_30s->65C_30s,-1C/cyc->72C_30s)x10->(94C_30s->55C_30s->72C_30s)x35->72C_4min->10C
|
| polymerase |
Toyobo rTaq DNA polymerase
|
| Genebank No. |
AB499501
|
| annotation |
|
| similar marker |
|
| product size in J |
180+190
|
| product size in Kaizuka-Wase |
185
|
| product size in Iki-White |
190
|
| product size in Tender Pole |
180
|
