+Marker Data: AFAT00C07

Crop bunching_onion
Population DJF2
Linkage Group Chr2b
Marker Type SSR
Map Position 54.0
Annotation
Source Name AB499441
Source Type genomic
Source Sequence
Developer NIVTS
Document Tsukazaki et al. (2007) Euphytica 157:83-94
motif (tg)5cgtgagtgcg(tg)3tt(ta)4
PCR product (bp) 113
fwd primer AFAT00C07-U
fwd primer seq. atgcacatcgcctagaatagcacc
rev primer AFAT00C07-L
rev primer seq. gtttctcaacatttggagtcagccc
fluor. primer AFAT00C07-F(PET)
dye PET
fluor. Primer seq. PET-atgcacatcgcctagaatagcacc
PCR condition 94C_2min->(94C_30s->65C_30s,-1C/cyc->72C_30s)x10->(94C_30s->55C_30s->72C_30s)x35->72C_4min->10C
polymerase Toyobo rTaq DNA polymerase
Genebank No. AB499441
annotation
similar marker
product size in J 120
product size in Kaizuka-Wase 120
product size in Iki-White 100
product size in Tender Pole 90
Line allele-1 allele-2
C1s-11s-25s-7 105 129
Cho1s-1s-2s-2s-8s 89 89
D1s-15s-10s-8 105 105
F1s-7s-17s-3s-1 - -
J1s-14s-23s-9 129 129
K1s-5s-2s-2s-4s-5 89 89
Chiang_mai 96 100
Kaizuka_Wase
Population Linkage Group Position
DJF2 Chr2b 54.0
DB Program Definition E-Value
EST Name Species Strain