+Marker Data: AMS14

Crop bunching_onion
Population DJF2
Linkage Group Chr2a
Marker Type SSR
Map Position 52.7
Annotation
Source Name AMS14
Source Type onion_gDNA
Source Sequence
Developer
Document Fischer and Bachmann (2000) Theor Appl Genet 101:153-164
motif (ca)28(ta)4
PCR product (bp) 169
fwd primer AMS14-F
fwd primer seq. cccctgagtaaattcaaaatcc
rev primer AMS14-R
rev primer seq. tccttagtataatttcggggtaac
fluor. primer AMS14-F(FAM)
dye FAM
fluor. Primer seq. FAM-cccctgagtaaattcaaaatcc
PCR condition 94C_2min->(94C_30s->60C_30s,-1C/cyc->72C_30s)x10->(94C_30s->50C_30s->72C_30s)x35->72C_4min->10C
polymerase Toyobo rTaq DNA polymerase
Genebank No.
annotation
similar marker
product size in J 150
product size in Kaizuka-Wase
product size in Iki-White 160+170
product size in Tender Pole 140
Line allele-1 allele-2
C1s-11s-25s-7
Cho1s-1s-2s-2s-8s 144 144
D1s-15s-10s-8 150 150
F1s-7s-17s-3s-1
J1s-14s-23s-9 154 154
K1s-5s-2s-2s-4s-5 144 144
Chiang_mai
Kaizuka_Wase
Population Linkage Group Position
DJF2 Chr2a 52.7
DB Program Definition E-Value
EST Name Species Strain