| motif |
(tca)14
|
| PCR product (bp) |
158
|
| fwd primer |
ACE111-F
|
| fwd primer seq. |
acttggatgtagaaacttcacaacatt
|
| rev primer |
ACE111-R
|
| rev primer seq. |
ttgacctaacaaatatagtcccacaaa
|
| fluor. primer |
|
| dye |
|
| fluor. Primer seq. |
|
| PCR condition |
94C_2min->(94C_30s->60C_30s,-1C/cyc->72C_30s)x10->(94C_30s->50C_30s->72C_30s)x35->72C_4min->10C
|
| polymerase |
Toyobo rTaq DNA polymerase
|
| Genebank No. |
CF440609
|
| annotation |
weakly similar to GP|17979475|gb|AAL50074.1||AY070733 AT3g25150/MJL12_9 {Arabidopsis thaliana}, partial (8%)
|
| similar marker |
ACM177
|
| product size in J |
150
|
| product size in Kaizuka-Wase |
130
|
| product size in Iki-White |
150+170
|
| product size in Tender Pole |
|
