Typing_Method |
Tm-Shift
|
Marker_Class |
SOL
|
STS_primer_fwd |
GCCAGAGGGAATAACAGCTGAGAA
|
STS_primer_rev |
ATCCAGCTTCTTGTAATGGCCTTG
|
amplicon_seq |
NCATGGACACTCTTTCTGATGGTGCACCGAAACATAAATCTTCATCATTCTTTCAAGAGGAAAGTGAAAGCTCTGTTACTACTCAACTCAATAGGCTATTTGGACGTCAGAAACCAATCCACCATTGTTTGGGTGGAGGCAAATGTATGGTAGTTTATTCATTATCGTCTTTATCTCATTATGGCTGTATAGCAATGCTCAATATGTTTCCTTGGATTTGTAGCTGCTGATAGCATGTTGTGGAGAAATAAGAAAATTTCAGCCACCGTTTTAGTCAGTGCTACAGCCATCTGGGTGCTATTCGAGTGGCTCAACTACAATTTTCTTTCACTTATGTGTTTTGGTCTGTCCTTTGGTCTGATTGCGCAGTTTCTGTTGAAAAATGCTTCTGGGGTAGTAAACAGGTACTTTTATTACCCGTGACCATCCATTGTTTATTATGCATAGGTGATCACGCTATTTTGATGATGTTACTGTCCCTTGCCTGTATCTTCTAGACTAGTTCCTATTAATTTTCTCTTAACACGCTGAAGTATTGAATATTTAAGGATATCAGGATGCAAAATTGCAAACAAACAAGTGTTGAACGCCGTCTCCCAAAGAGTGTCCTTTTTCGTCTACTGTATTGGTGTAGAACTTCATAAACATTCTGTTCAAACACGGTTCACTATAGTTAGTCTCTTTTCCATTTTTATTCATCTCTTTCTGCTCCGTATATGCTTTGATATATAACTTAATTTCAGTCGAGGCAGAGTGAAGTTTAATGTAGNACTAGCTGGNAGTCGTGGGTCTAGTTATGATACCTAAATTNCTGAATATATCTGGCATAACAGACTCCGCGGAACATAGTTGAAGGGGTTTAGNTTACCCAAACTATTATCGATAGAACGAGAAANCCAAAATAGTGGCAGGTTGTGTATTTTCTAAAGCAATGTTCTCTTCTTGTCTTTGTNATTCTAATTTGTGTATTATATATCACTATCTTGAATCAAATTAACGGCAGTTCATTCTATGGCNNNNNNNNNNNNNNNNNNNNNNNTGGNTTGGGGTTCCTTCAAGATGCTGCCTTGGGCGGTAACCTGAAACAATTTGTAGTGGTATGCTTAAAGTCATTAACCATTCATATCTATCCTCAGTGATATGCTTACAATAAAATGATACAAAGTATCAATTACTTGATGTGAATTATGTGGCCCTTTCTTTGAACGTTGTAGCCTTTTTATTTGATTATCTTTTCACTAGGTATGAGGTGTGACATAAGTATGAACTGTGTCCGATCATAGACCACCAGGCAATACAAGGCTAAGTGACTTAGGTTTGCTTCTATAGGCTAATCGAGATGGAATTTCCCTTTGAAACTCAATTACGAGATCCCAAGACTTGTGCCATTGTTTGTTCAATATTATGTTCTCGCCTTACTTATTTTCATGATCTCTACTGAGATTTTCGAACTAAGATCCATAAATTCAAAGCTCTGATCACCAAATTTTCTTGCAGCTGTTTGACATCCCCCCTCACAAAAAGACTTTTCCCAATTTCAGATGAGATTTTTCCATGTTTACATCTTCTCTCCTCTCTATGATGTCAAATTTCTTTTACTGTAAAGTACACGAAGCTAATGTCTTGGGATTTGTCCTAGGTTATACTGAGCTTGTGGGCTGCTGGTATGATAGGAAGCTGGTGCAACTTCTTGAGTGTTGTGTATATTGGTAAGAGCTCTCTTTTCCATGATAGCGTACTACCATTTATCTTCGAGTATCAAGCAGGGGAGATCATTTCATTCTACAGATAGCAATTTCATACTGAGATGAAGATGTCTAACGTAGGAGTATCAACGTCATCATGTCACTTATAGATACAAATATGCAANTAATCTTGTCTCCAGAATTGTTGCCACACTCGTGTCTGACATTTTTGAAGAGTCATATTGATATCACCGTGTCAATCCCGACTAACTTTGAATTTTGATTTATAAGGATTCGTGGCTGCTCACACTCTTCCAGTGCTATACGAAAGATATGAAGATGAAGTTGATAGTTCGTATACAANN
|
SNP_position |
1778
|
allele_1 |
T
|
allele_2 |
C
|
ASP_primer_S |
GCGGGCTCGAGTATCAAGCAGGGGAGATCATTT
|
ASP_primer_L |
GCGGGCAGGGCGGCTCGAGTATCAAGCAGGGGAGATCAGTC
|
Common_primer |
CTCCTACGTTAGACATCTTCATCTCAG
|
enzyme |
Stoffel frag.
|
dye |
EvaGreen
|