Typing_Method |
Tm-Shift
|
Marker_Class |
SOL
|
STS_primer_fwd |
CATCCGTACAGTTGCTGGATTTGA
|
STS_primer_rev |
TCAGTTAGGTCATCCAACCAACCC
|
amplicon_seq |
TTTGATCTAGAGTTTTTGCCTTCTGATTATCTTTCGAAAGAATTTTACTATGTTCTATTTAGAAGCCACCTTTAAGTTTTCAGGCGCTTCTAATTAGGTATTTTACATATGTACTATTGAGTTAAGAGTGAATAGGTTAAGACCCCCTAAGCTATCATTTTGCGAGTTTCAACATGAACTGTTGAGGTCCCCCCTCCTCTCTCTCTATACTATTATGAACATATATTAAAAATACACTCCCCAGAGGAATATGACGTGCTTACATGAATATCTCCAAAAGCTCCTCCAAAAGAGTTCCAAGTAGCAGTATACGTTTTTTTTTTTTTGAGTGAATAGGTTAAAAAACCCTTCCCTCAAACTATCGCCATTTCATACCTAAACTATTGGGCGTTCACAGCATCCCCAAACTACTATGAAGTCATTCTTTCCATCATCTGTCACATGCCTCTGAACAAGTTATCCGAACATTTTGCCGCATATTCAAGCGGTAGTTTTTTAATCTGAAGGACGTTATACTCCAGGGGATGATAACATGAACACCCAATAGTTTAAATATGAAACTTGCAAAATGACAATAGTTTGGGAGGGGGTGGGAGGGAGGGTGGTCCTTGGGGATGTACCCCACCATGTTATCTCAATTTATCTCATTGCTGTTTGGTATTGAACAGCTCTGTTTTGCTGCTAAAACCTGGTATGGGGGGTTTTTGGGCATGATGGTTTTTGGTGCAAGTGAAGTGAGCAGAAATGTGGACCTTGGCGATGAAACCACCACAATGATGTGCAAATTTGTTATCCGAGCATCAGACGAATCCATACCAAGGAGATGCTA
|
SNP_position |
405
|
allele_1 |
A
|
allele_2 |
G
|
ASP_primer_S |
GCGGGCCTATTGGGCGTTCACAGCATCCCCA
|
ASP_primer_L |
GCGGGCAGGGCGGCCTATTGGGCGTTCACAGCATCCCCG
|
Common_primer |
TCAGAGGCATGTGACAGATGATGGA
|
enzyme |
Native Taq
|
dye |
SYBR Green I
|