Typing_Method |
Tm-Shift
|
Marker_Class |
SOL
|
STS_primer_fwd |
TGAGCTCCAGATTGGGCTTAAGAA
|
STS_primer_rev |
AGGACCCAAGAGACGAGGAATCTG
|
amplicon_seq |
TCAGATTCTGAGCCAGAAGCATGGTATTTCTTTGCAAGCTTCTTAACTAACTTCTTGTTCTTATTAAGCTTCTTGAGGCTCTCAACATCCATGTACTCCAAACCAATCTTTTCTGCCTGCAAACACATGCGCCATGTTCTACTTATCACCCAAGAAACACTAAAGATTCAGGAATGATTCACCTAACATTAACTTTTAATGTTTTTTTATCATTAGTACCAACAAATTCACCAAGCAGAAAATATAAAATAAACATCATAAAACTAAAAAGTAGACAATCTACTAATAGGCATGCAACCTTGCAGGAGTAATAAGTAGATTTACCCATTGCAACTAATGGGACTTCTAATGACATTGCATCTTTAAACCAAGTTAACATATTATTGAGCTGTCACACAATTATTGCATAAGGAAATGGCAGACTCGCAGTTGACAGACAAGCTACAAAATATCCCATATACCAGAACATTAAAACCATATTTTGATTGTGACTCAACTCTATCAACTGCTACTTAATTGCTAGTTGGTCAGAACTGATGATAACATCGTTTCGCCCATCGCAATTACAACACATAATATTCATTCTCTTTCAGTTTTATCAATTAATTGAAGAATTTGTCCAATAAAAATCAGTCTTCTATCAATTAAAGGGGTGATGATAAGATAACCATGACACAAAAATGCATCTTCTAGTAGTGAATAGAAAAACCTAATCTTCTATACTAAAACAACAATATTTAAGGGATGCAACAGAAATCCGCTCAGCAGTTGCATATGCACTTATCAAATAATGTTGTGGTTCCCAAAAAAAAAAAGACACAAATCCAGCATAGCTAGCGACATCAATTAGCATGGAAACAAAGATTACCTCCTCCACGTGCTGAGCATCTCCGAGCATGCAAATCTTCATCTTAGGGCGTGGAATATGAGGCAACTTAACAGATCCACTAAAACGCTGACGTTAGT
|
SNP_position |
652
|
allele_1 |
G
|
allele_2 |
A
|
ASP_primer_S |
GCGGGCTTGTGTCATGGTTATCTTATCATCACT
|
ASP_primer_L |
GCGGGCAGGGCGGCTTGTGTCATGGTTATCTTATCATCCCC
|
Common_primer |
TTGAAGAATTTGTCCAATAAAAATCAG
|
enzyme |
Native Taq
|
dye |
SYBR Green I
|