| motif |
(ct)9at(ct)8
|
| PCR product (bp) |
142
|
| fwd primer |
AFB221-F
|
| fwd primer seq. |
acgacaggttgtgtgattgtgtg
|
| rev primer |
AFB221-R
|
| rev primer seq. |
gtttgaccgatgtaggatgattgtt
|
| fluor. primer |
|
| dye |
|
| fluor. Primer seq. |
|
| PCR condition |
94C_2min->(94C_30s->65C_30s,-1C/cyc->72C_30s)x10->(94C_30s->55C_30s->72C_30s)x35->72C_4min->10C
|
| polymerase |
Toyobo rTaq DNA polymerase
|
| Genebank No. |
AB499493
|
| annotation |
|
| similar marker |
|
| product size in J |
120+130
|
| product size in Kaizuka-Wase |
120
|
| product size in Iki-White |
110+120
|
| product size in Tender Pole |
90+120
|
