+Marker Data: AFS142

Crop bunching_onion
Population DJF2
Linkage Group Chr1a
Marker Type SSR
Map Position 133.3
Annotation
Source Name AB499335
Source Type genomic
Source Sequence
Developer NIVTS
Document Ohara et al. (2005) Euphytica 144:255-263
motif (ac)3aa(ac)11a(ta)4tgt(ta)8
PCR product (bp) 250
fwd primer AFS142-F
fwd primer seq. tgagagaattaatattattggaggcctat
rev primer AFS142-R
rev primer seq. ataaaatgacaaccaacccatgtta
fluor. primer AFS142-F(FAM)
dye FAM
fluor. Primer seq. FAM-tgagagaattaatattattggaggcctat
PCR condition 94C_2min->(94C_30s->60C_30s,-1C/cyc->72C_30s)x10->(94C_30s->50C_30s->72C_30s)x35->72C_4min->10C
polymerase Toyobo rTaq DNA polymerase
Genebank No. AB499335
annotation
similar marker
product size in J 250
product size in Kaizuka-Wase
product size in Iki-White 250
product size in Tender Pole
Line allele-1 allele-2
C1s-11s-25s-7 248 248
Cho1s-1s-2s-2s-8s 252 252
D1s-15s-10s-8 246 246
F1s-7s-17s-3s-1 248 248
J1s-14s-23s-9 244 244
K1s-5s-2s-2s-4s-5 248 248
Chiang_mai
Kaizuka_Wase
Population Linkage Group Position
DJF2 Chr1a 133.3
KiCF2 1a 153.7
P1 J02 94.9
P2 D02 102.9
SaT03 1a 104.2
DB Program Definition E-Value
EST Name Species Strain