+Marker Data: AFB01B03

Crop bunching_onion
Population DJF2
Linkage Group Chr1a
Marker Type SSR
Map Position 161.2
Annotation
Source Name AB499467
Source Type genomic
Source Sequence
Developer NIVTS
Document Tsukazaki et al. (2007) Euphytica 157:83-94
motif (ag)11
PCR product (bp) 215
fwd primer AFB01B03-U
fwd primer seq. atttgcattcgatttcgttgagtg
rev primer AFB01B03-L
rev primer seq. gtttcagcacacttcgtttggatttcag
fluor. primer AFB01B03-F(NED)
dye NED
fluor. Primer seq. NED-atttgcattcgatttcgttgagtg
PCR condition 94C_2min->(94C_30s->65C_30s,-1C/cyc->72C_30s)x10->(94C_30s->55C_30s->72C_30s)x35->72C_4min->10C
polymerase Toyobo rTaq DNA polymerase
Genebank No. AB499467
annotation
similar marker
product size in J 240
product size in Kaizuka-Wase 240
product size in Iki-White 240
product size in Tender Pole
Line allele-1 allele-2
C1s-11s-25s-7 204 204
Cho1s-1s-2s-2s-8s 220 220
D1s-15s-10s-8 200 200
F1s-7s-17s-3s-1 206 220
J1s-14s-23s-9 204 220
K1s-5s-2s-2s-4s-5 206 220
Chiang_mai 204 204
Kaizuka_Wase
Population Linkage Group Position
DJF2 Chr1a 161.2
KiCF2 1a 185.5
DB Program Definition E-Value
EST Name Species Strain