Typing_Method |
Tm-Shift
|
Marker_Class |
EST-misc
|
STS_primer_fwd |
GCGGAGAAACTGGCTAGGAAGAAA
|
STS_primer_rev |
CCTTCGATTCACTTCCTTTTCCAA
|
amplicon_seq |
AAATCTTCAGTTCCTGCATCATTAGTCCACTGTTAGTATTTCCTCTTATGTACTAGCTACTTGTGTTTACTGTAGAAACAGTGTTATCAACACGAAGGATAGTGTAAAGGCACCTTAGGTCCTAAGAACAAGCCATAGGGGACACCATTGAACTTCTCTGAATGATGAAGCTGCAAATTCAGATATCAAACACATGCATTAGAAAATCCTACCTACTGATGTATTACTCAACGGTCGCACTAAGTGTGATTAAATTACCGAATGAGCTGCAGCCACCTTCCTAAGATAAGGTACATTGGCTACAGGTCCAACCGGGAATCTCTTGTGAACCAAACCATCATGAACGAACATGTATGCCATCCCAAATACTGTGATCCCTAGCCCCTGCATAAAACAAGAGTTCAGTTTCATCGACAGTTATCTTAATCCAGTAACATTTCTAGACTATCCTAGGGAAAGATTTAGAGCATGATCGTCATTGCTCTCAATTCGAATCATTTACACTATATCATCAGTATATAAATATGGATGACGTCCATTATGAACTAACAAATTCGTTCTGCTACAGATCATACTTAACATTACAAAAGAAAAGACGGAAAGTTAAATGAGAGCATACAGCGCCGAAGCAGAGGCCGGGAATGAGGCCTTTATGGAAGAAACCATAGTCGAGGAGGGCTATTGCTGGAACAGCATTTATTATGGCGAAAACATCATTCAGCTCAAAAGGTCCTTCTCTTGGTTTGTGGTGTGACTGCAACAATTTTAACGTAACAACAATCTTAATATAAAGTTTTCGTCACAAAGGATGAAATGAGGCATTAACTAAAGCTCCAAATTGTAGAATTATACTGTAAGTAGTACACAAACCTCGTGCATGTGCCATAGAGAAGCGTGCCACAGAGCTTTATGTGCCCACCTTGCCCAAAACTCCATTCCTACCTGAAATTTCCAATTTAAAATCAGTCAATTCTGCTATTTCTAGTTACTCTCTTGGAACAAAGTTGTTTCACTCTCCAACATAAAAGCGCCTTCCTATGAAGATCGAAGATAGAAGGCAGAACTTACAGCAGCACCAACAGCGAGCGCAAATGTACCCAACATTTCGGATAAAGGAACTTCTCTGCCCTACACCAAAGAGAGAGAGAGAGAGAGAGAGAGAACTCAGTAAGAAGTCAGTTTCGAAAAGAAGAAAAAGAGTAAAGTGCGAATTACTGTATGAACTTTTCATGTACCTCCATTTGCCATGAAAATCTGTAATAAACCGCCATAACAGCCATGGAAGTAATGCCAAAACTAGACATAACAGCAGCCACAAGATAAGTACT
|
SNP_position |
239
|
allele_1 |
A
|
allele_2 |
G
|
ASP_primer_S |
GCGGGCACTGATGTATTACTCAACGGTCGCA
|
ASP_primer_L |
GCGGGCAGGGCGGCACTGATGTATTACTCAACGGTCGCG
|
Common_primer |
GTGGCTGCAGCTCATTCGGTAATTT
|
enzyme |
Stoffel frag.
|
dye |
EvaGreen
|