Typing_Method |
Tm-Shift
|
Marker_Class |
EST-misc
|
STS_primer_fwd |
TGAGATGAGTACTGCTGGTGATCG
|
STS_primer_rev |
CTTCACTAGATGAGTAATGCCAACATC
|
amplicon_seq |
GAAATGTGTTTCTTTAACTTAGGGACGTAGGTTGTTTGTCTAATATTTTATTCTTGCAGCCAAGATATCACAATGTGAAGCATATCAACCTTGAGTTTGCGCAGGATATTGAAGACAAGCAACTAGAAAATGTTAAAAGCAAGGTGGGATTCATAAGTTTGGAAAGTCACAGAAATTAGCATGTTTTCAGAACCTTTTTTTTTTTAAATTTCCAATCAGTAAAAATTCCAAGGTAGAGTCTGCCTTTAATTAGCACCTCTGGCTTATTTTTGACTTCGTATCAATATCTACTTGGAGCCATTTTATAATCCTCAGAAAAGTTAGAAGTGATTTTTTCTTGTTGTCTCAACAACATGGGTTACCTGTATATAGAGGTGTCAAAAAATAAGAAAAATATTCATATCTTAGCATCTAATGAAGATAAAGAAAGTTCGCCTAATGGCCTAATCCTTGTAATTTGAGGTAACAAATTACTCTTAATGTGTCCTGCATGGTACTACTTTTGTCACAAAACCCTTGAGATGAGGTCTGCTGGTGATCGTCTTGTTTCAGCTCTTTCTTTGCCGAGATATCGCAATGTGAAGCATATCAACCTTGAGTTTGCGCAGGATATTGAAGACAAGCAACTAGAAAATGTTAAAAGCAAGTGTGGGGATTCCCTTTTAGATTTGGAGATTTTAAATCTAAATGGTTGTCAAAGGATCTCTGACAAGGGAATTGAAGCAGTGAAAAATACTTGTCCTAAACTGAAGGTCTTTTCCATTTATTGGAATGTAAGGGTAACAGATGTTGGCATTACTCATCTAGTGAAGAGTTTTCCACCATAAATGATTTCCTAGAAAATGTTATTATGGAAAACAAGTGATTTTTTAAACTTGTTTTCTCGTGTATGGTATGTAAGAAAGAAAATATTATCCCAAGAGCTTTTATATGTTATCTAGCAGGATATCATGAATACGGGATGGGATGGGGAGTGGGGTTTCGGAGGTGGGATGGTCAATTATTGGGGCTTGTGTGGGTGGGGAGAAGACAAGAAATATGGAGTGCTACTTGTTTTCACTGAAAACCATTTTCCTCATTTTTAGAGAACTTGTTTTCCTAGAAAAAATGGTTTCCAAAGATTTTGACCAACCAACCATAGGAAAATCGGACAACATCTTTTGTTTTCCTCCATACCAAATGCATATTGTGAAACTGTAAGCCTGCCTCGTTGGTTATATATATATATATATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTTTGTTTTCTTTTTAGCAAG
|
SNP_position |
390
|
allele_1 |
A
|
allele_2 |
G
|
ASP_primer_S |
GCGGGCATTAGATGCTAAGATATGAATATTTTT
|
ASP_primer_L |
GCGGGCAGGGCGGCATTAGATGCTAAGATATGAATATTGTC
|
Common_primer |
GTTGTCTCAACAACATGGGTTACCTGT
|
enzyme |
Native Taq
|
dye |
SYBR Green I
|