Typing_Method |
Tm-Shift
|
Marker_Class |
SOL
|
STS_primer_fwd |
TTCCTCATGGGGATTCATCAGTTT
|
STS_primer_rev |
ATGAGACAGCCACTTCATCAAGGA
|
amplicon_seq |
TTATTCAGCCTTTTGACGAACTTTTTGTAGATTTTCAGGTATTTGATTGGTGTAAAATTCTTCGACATATTCTGATGTACTAGACAAAGTTTCAACAATTGATCATATGTACTTGTAGGAGTTCGATGTGGATGCTTTGGCAAAGTTGGTCGAAGAAGCAACTATTCCTACTGTGACTGTATATAACAAGGACCCAAACAACCATCCATTTGTTATTAAATTCTTCAACAATCCAAATGCCAAGGTGCAGCATTTNCTTAGTCTACGAGATTTATGTTGCCTTTTAAGATTCTGATAGTCTTCTTGCTGTCTAAACCTGCATCTACTTTCCCATACTTTCTGTTGATAGATCTCTTTAGTTGGAATATATAAGAAGTGATGTACACTAGACAGNTGAATNACATAATGCATGCTATTAATTCTCTTACTTGGTGTCTCTTGTCCTCCATTTTGGAGGAACATTGTTTGATATATTAGAAGATTAAGAGCCTGTTTGGATTAGCTTTTGGCTTATGACTTATAAGTCAAAAGCCATAAGTTAGGATTTCTAACTAACGGCTTTTGGCCTGTTTTTGTTGTTTTGCCTTAAAAATAAGGATTTATGGTCTTCCATTGCGTTCGTTCTTAGATGTTATTTCNNNNNAGCAATAAGCATGAACTCTTCATGTATAATATTTCTAACTAANCGGCTTTTGGCCTGTTTTTGTTTTTTTGCCTTAAAAATAAGGATTTATGGTCTTCCATTGCGTTCGTTCTTAGGTGTTATTTCATTTCTGCAATAAGCATGAACTCTTCATGTATAATAATAAAGCGCCACTAGCAAAGATAGATGTATAAAGATTGGTGCAAGATTCATCTGTTTTTTTCCTGAAAGTATCGTTGTTCCTCGGGGACCTCCAGAAAAGATTGANGTTTTCAGTGTTTTTAGCCAATAAACTTGTTTATCATGGCTGCTGCTTGTTTAATATCCTTTTAGCATGTACTTTNCTAGANGCTGAAGTTAAGAATNNNNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNGAAGCCGNNNNNNNNNNNNNNNNNAAATGTTGAGCTGATCAANTTGCTTCATGGATTAAGGAGTTTAAGNNNNNGNNNNNNTTCTCCTGCNTTCACTTTTTTCATTTACCNTTTTCTCTTGGTTTAATCGTTTCTCCCAGGTTTTCTCATCCATTATCTATATATATTCATCTTATATATATCCCTTTGGACATTTTAAATGGACGAGTTCTCCTCTTACATTCAAAGTTATACTTAATCAGTAGCAAATTGAATGTAAAGAGAAGTCCTCAAGAAACATTGTTTCTAGGGAAAAATTGTTTCTTGTACTGTTTCTGAAGTAATTACTTGATCATACTGTAGGACGGCAAGGTGAAACCTTTCAAAAAATCAGAACCTATCCCAGAGGTTAACAGTGAACCTGTTAAAGTTGTGGTTGCTGACAGTTTACAGGATATGGTTTTCAACTCTGGGAAGAATGGTATTTCCTACCTACTGTTCAAATTTTACTGACATTTTGTCATTTCCATCTTAGGCAGCTCATATAGATTTCATTGTCTTACTTCTCATGCATTCTTGTGTGGTGTAAGTATTTGAAAATGAAATTGTATATTTCATCTTCTTGTATGGCTCTATAGCATGACANTCATATATTTGAAAAGCATATACACCGACTTACTNCAGTGGTTATTTGGAGATAAGTTGTTCCAACTNNNNNNNNNNNNNNNGTTCAAGAATGTTGCTAAGTAGCAGTATCAAATATATGCTATAAATTCAACATCTGCGGAATAGGAGACGGTATTAATTAAACAAATTGCATCACAATGTATTTACATGCAATATNCCATGAAGTTATGAGTCAATTATGTGGCGTAGTTCAATTTCTTCCTTCATCTTAGTTGGGTGTATTATCCTTGTGTTCNTCTTGCTCGTCTTCCTGAACTTGTACATGGTCTTTTGTTAGAATATTTGTTGGATATGCAGCAATAATAATTCATGTGATGGTTTTTTCTTATAGTACTCCTCGAGTTCTATGCCCCTTGGTGTGGACACTG
|
SNP_position |
1862
|
allele_1 |
A
|
allele_2 |
G
|
ASP_primer_S |
GCGGGCATTGCATCACAATGTATTTACATGCAA
|
ASP_primer_L |
GCGGGCAGGGCGGCATTGCATCACAATGTATTTACATGAAG
|
Common_primer |
TGAAGGAAGAAATTGAACTACGCCACAT
|
enzyme |
Stoffel frag.
|
dye |
EvaGreen
|