+Marker Data: emk04N11

Crop eggplant
Population AL2010
Linkage Group E_06
Marker Type SSR
Map Position 37.9
Annotation
Source Name emk04N11
Source Type genomic
Source Sequence ACTTGTAATTAATTATCTATATTTATACATATCATTTTCA TCCCAATTTTACTTTCAAAATTAAACTCCCCCTCAACTTT GAACAATAATCAAACTCCTCCTTTATATAATGCATTGTCC ATTTATCCTTGTTATATCACACACACACACACACACACAC ACACACACGCACACACACACACATATATATATATATATAT AGAATTCTCTTTATTTCAAAATAAGTGAATTGTTGGGCTA TATCACACATATTAAGAAAAAAAATAAAGACAAAATTTAA TCATATTTTTTCATTTTTATCCTTTTCAAACTTCAAACAC TAATTTAATGCATCATTTATTTCTAATACAAAATTCAATC CTAGAAACTCTGGAAGAAGATAATTAGGTAGAAATTAGAT AAGTAGTCATAGTCTTAAACACAATTTAAATCCCAAAACC TAGTGTCACAAGT
Developer NIVTS
Document Nunome et al. (2009) Development of SSR markers derived from SSR-enriched genomic library of eggplant (Solanum melongena L.). Theor. Appl.Genet. 119: 1143-1153

motif (ac)15gc(ac)6a(ta)9
PCR product (bp) 186
fwd primer emk04N11/U
fwd primer seq. aactccccctcaactttgaacaat
rev primer emk04N11/L
rev primer seq. tgtgtgatatagcccaacaattcac
fluor. primer emk04N11/U_FAM
dye FAM
fluor. Primer seq. FAM-atctccccctcaactttgaacaat
PCR condition 94C_3min->(94C_30s->65C_60s,-1C/cyc->72C_60s)x10->(94C_30s->55C_60s->72C_60s)x30->75C_5min->10C
polymerase Roche Taq

Line allele-1 allele-2
AE-P03 190 190
EPL-1 188 188
LS1151 (S.linneanum) 183 183
LS1934 188 188
LS4021 (S. incanum) 148 148
Nakate Shinkuro 182 182
Senryo 2 186 186
Shironasu 178 178
WCGR112-8 194 194
White Egg 180 180
PI 286106 184 184

Population Linkage Group Position
AL2010 E_06 37.9
ALF2 AL06 43.4
EW2009 LG04 66.9
LW2010 E_06 35.6
LWA2010 LWA_06 36.514
LWAE2012 E_06 43.119
LWF2_2022 E_06 41.9
N28F2 E_06 50.4
NAF2 NA06 38.9

DB Program Definition E-Value
TomatoBAC_end BLASTN SL_EcoRI0092L23_T7_356626 Chromat_file:SL_EcoRI_092_L23-T7 SGN_GSS_ID:373452 (vector and quality trimmed) 3e-07
TomatoBAC_end BLASTN SL_EcoRI0129N10_T7_334005 Chromat_file:SL_EcoRI_129_N10-T7 SGN_GSS_ID:350831 (vector and quality trimmed) 3e-07
TomatoBAC_end BLASTN SL_EcoRI0123D13_SP6_329244 Chromat_file:SL_EcoRI_123_D13-SP6 SGN_GSS_ID:346070 (vector and quality trimmed) 3e-07
TomatoBAC_end BLASTN SL_EcoRI0037D15_T7_281157 Chromat_file:SL_EcoRI_037_D15-T7 SGN_GSS_ID:297983 (vector and quality trimmed) 3e-07
TomatoBAC_end BLASTN SL_EcoRI0037D13_T7_281155 Chromat_file:SL_EcoRI_037_D13-T7 SGN_GSS_ID:297981 (vector and quality trimmed) 3e-07

EST Name Species Strain