+Marker Data: emk03O04

Crop eggplant
Population AL2010
Linkage Group E_06
Marker Type SSR
Map Position 35.1
Annotation
Source Name emk03O04
Source Type genomic
Source Sequence ACTAGTAGCCTTTTTTTTCTTTTTGATAATCATGGTGTCG GAGTTAGAAGTTAGACCTCTACGTGCCAACTCCCACCAGT ATAAGTATCAAAAGAAATGGCTGTGCTTTTTTGACTTTGC TGAAATGTGAACTTGAGAGCCTTTTTGCTGCTAAGGTTAG TAGCTTGACTAATTATACAGAATACATGCTAACTCCGACC AGTACTTTTCACTACTAAAAGTCCTCCAAAAATTCTACCA AAAATTCGGCAGCATAACCCCTGTAAAATGGACTATCCCA GGGGTTTCAAAGCTGCCCCTTAACAGCCAACAACATCACA ACAAGTCCCCATAACAATTTCCAACAATAACAAGGCTATA GGGTACTACCCTTATTATTAAATAATGTGTTTACTTTCAA AAAGGTATTCCCCTGAGATGATATCAACCCCTACTTCACA ACTCTTTGACTTGAATTTTTGTCGTTAGAGGAATCGATTT TCATGAATCACAAAAAAAGGGAATTAGGGAGGGGCCTGTC TTAGTGTAATTTGTTTTACTTTATGAAATTTTATCTATTT TTCAAGACTTAATAATATATGTTAAGGGGTGATTAAAAAT AAAAAACGAATGAAAAAAAAAGATTTGGGCAGCCACTTTT GTATAGCCTTTCTAAGTTATTTTTAACATCATACATACAT ACACACACACACACACACACACACACACACACACACACAC ACACACATATATATATATATATATACATATATATATATAT ATATATATATATTGCCCTAAGTTTAGTTGGTTCCAAGACT TATACACACATTATAAGCTCCCTAACAGTTTTATATTTGA TTAAATGATGAAAACAATCAATCCCTTTGAGAAAAAATCA ACAAACCCCTATCATTTGGTAAGTAGCTAAATTCTATCTT GAAACTGTGTAATGTTTTGTAATTGTGGAATATATCATTG CATATGTCTGAGTTTGTAGTTTGTAAATATGTTTTGGAAA GCTGATTCACTTATCCACAACCTTTATGATTATGTGAAAT TATGATGTGCTTGTATGGT
Developer NIVTS
Document Nunome et al. (2009) Development of SSR markers derived from SSR-enriched genomic library of eggplant (Solanum melongena L.). Theor. Appl.Genet. 119: 1143-1153

motif (ac)23a(ta)9ca(ta)12
PCR product (bp) 177
fwd primer emk03O04/U
fwd primer seq. aagatttgggcagccacttttgta
rev primer emk03O04/L
rev primer seq. ttggaaccaactaaacttagggca
fluor. primer emk03O04/U_NED
dye NED
fluor. Primer seq. NED-atgatttgggcagccacttttgta
PCR condition 94C_3min->(94C_30s->65C_60s,-1C/cyc->72C_60s)x10->(94C_30s->55C_60s->72C_60s)x30->75C_5min->10C
polymerase Roche Taq

Line allele-1 allele-2
AE-P03 176 176
EPL-1 176 176
LS1151 (S.linneanum) 125 125
LS1934 150 150
LS4021 (S. incanum) 195 195
Nakate Shinkuro 178 178
Senryo 2 168 176
Shironasu 180 180
WCGR112-8 174 174
White Egg 172 172
PI 286106 149 149

Population Linkage Group Position
AL2010 E_06 35.1
ALF2 AL06 40.4
EW2009 LG04 71.2
LW2010 E_06 35.6
LWA2010 LWA_06 35.455
LWAE2012 E_06 42.23
LWF2_2022 E_06 39.7
N28F2 E_06 48.9
NAF2 NA06 38.9

DB Program Definition E-Value

EST Name Species Strain