+Marker Data: emg21M05

Crop eggplant
Population AL2010
Linkage Group E_06
Marker Type SSR
Map Position 38.6
Annotation
Source Name emg21M05
Source Type genomic
Source Sequence CCTAACACAAGTAAGAAATTATAGTTCTTGCCATATGTTG TTTATTATGTTTTGGTTATCATACTATTTTGTTATTGTTA TTGTCTCTTTCTGGTAGATGTTTCACTACTTTTTCTTATT AACAACTATGCTTTATTCATTGTCTTCTTCTTCCTTTACT TGGGTTTGATGCACTTGAGCCGAGGGTCTTTCAGAAACAG CCTCTCTACCACCCGAGGTAGTGGTAAGGTCTGCGTACTC TACTCTCGCCATACCCCACTTAATGGGATTTCACTAGATA TGTTTTTGTCATGTAAATGTGATGATAAGTCATTAATCAT AGTTGCAGGGAGATCTTGGAACGTCTTTGTTTACTCAACA ACTTGCTTGGTGTTATTTGCATGCTTGTCACATATGCTTA GTTGCGCCTAATCTCTCTCTCTCTCTCTCTCTCTCTCTCT CTCTCTCTCTCTCTCTCCCTTTTTTGCTGAAGTAGGACGA TGAGAGTGCTCTTCTGCGTTTCCTAGAATGCAAGTTTGGT AAAGGACAACCAGGTGCTCCTGAATTCTTTTATGATCCCA AATATGCGCTACGCCTTTGCCTCAAGGAAAAGAGAATGCG AGCTTGTGTTCATATATATAGCATGATGTCTATGCATGAA GAAGCAGTTGCCCTAGCACTACAGGTTCTGTTAAATATTG ATTGCCCTCTGTGTGTTTCCAAGAAGCCTGATGGTTCTGT TAATCTTACTGCATATATTCTTTAATCTATGACTACATTA ACTATGAAGTTAGCTTATTTGTCATGTTGCATCCTTTACT GTCCTTTCGAATGAGCTGAAATCAAGAATGTAAGGCATAA GGTTGGTTGATTGATTGCTGCTTGTCACGTTTAGGACAAC AAGACTAAGAGCCAGTTGTCAAGTACAAGCTCTTTTTGTT ACTTGCACTAAATAAAAAGAAAAAAGAC
Developer NIVTS
Document Nunome et al. (2009) Development of SSR markers derived from SSR-enriched genomic library of eggplant (Solanum melongena L.). Theor. Appl.Genet. 119: 1143-1153

motif (tc)23
PCR product (bp) 153
fwd primer emg21M05/U
fwd primer seq. tgtcacatatgcttagttgcgcct
rev primer emg21M05/L
rev primer seq. agcacctggttgtcctttaccaaa
fluor. primer emg21M05/U_VIC
dye VIC
fluor. Primer seq. VIC-agtcacatatgcttagttgcgcct
PCR condition 94C_3min->(94C_30s->65C_60s,-1C/cyc->72C_60s)x10->(94C_30s->55C_60s->72C_60s)x30->75C_5min->10C
polymerase Roche Taq

Line allele-1 allele-2
AE-P03 151 161
EPL-1 150 159
LS1151 (S.linneanum) 151 151
LS1934 143 151
LS4021 (S. incanum) 154 154
Nakate Shinkuro 152 160
Senryo 2 156 159
Shironasu 151 154
WCGR112-8 147 156
White Egg 151 159
PI 286106

Population Linkage Group Position
AL2010 E_06 38.6
LWA2010 LWA_06 37.185
LWAE2012 E_06 43.776
N28F2 E_06 37.4

DB Program Definition E-Value
TomatoBAC_end BLASTN LE_HBa0015I22_SP6_129823 Chromat_file:LE_HBa_015_I22-SP6 SGN_GSS_ID:146654 (vector and quality trimmed) 1e-20
TomatoBAC_end BLASTN LE_HBa0108F06_T7_95867 Chromat_file:LE_HBa_108_F06-T7 SGN_GSS_ID:95867 (vector and quality trimmed) 1e-14
TomatoBAC_end BLASTN LE_HBa0077P05_T7_18777 Chromat_file:LE_HBa_077_P05-T7 SGN_GSS_ID:18777 (vector and quality trimmed) 1e-14
TomatoBAC_end BLASTN LE_HBa0201G20_T7_360123 Chromat_file:LE_HBa_201_G20-T7 SGN_GSS_ID:377334 (vector and quality trimmed) 2e-12
TomatoBAC_end BLASTN SL_EcoRI0025K05_T7_235762 Chromat_file:SL_EcoRI_025_K05-T7 SGN_GSS_ID:252588 (vector and quality trimmed) 1e-11

EST Name Species Strain